| 饲料锌添加水平对凡纳滨对虾免疫抗菌机能和溶菌酶mRNA及Toll受体mRNA表达的影响 |
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| 引用本文： | 郭腾飞,黄旭雄,苏明,王武刚,怀向军,胡盼,严佳琦,吕丹,黄征征. 饲料锌添加水平对凡纳滨对虾免疫抗菌机能和溶菌酶mRNA及Toll受体mRNA表达的影响[J]. 水产学报, 2011, 35(7): 1081-1089. DOI: 10.3724/SP.J.1231.2011.17264 |
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| 作者姓名： | 郭腾飞 黄旭雄 苏明 王武刚 怀向军 胡盼 严佳琦 吕丹 黄征征 |
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| 作者单位： | 1. 上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海,201306
2. 上海市青浦区水产技术推广站,上海,201701 |
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| 基金项目： | 上海市科委项目(08DZ1981000);上海市重点学科建设项目(Y1101);上海高校创新团队(第二期)建设项目资助 |
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| 摘 要： | 在基础饲料中添加不同水平蛋氨酸锌(添加水平分别为0、50、150 mg Zn/kg)并饲喂凡纳滨对虾,养殖14 d后,取样测定对虾鳃组织中Toll受体mRNA和溶菌酶mRNA的表达水平以及肝胰腺、肌肉和血淋巴中超氧化物歧化酶(SOD)和溶菌酶(LSZ)活性,并进行溶藻弧菌人工急性感染试验。结果表明,凡纳滨对虾肝胰腺及肌肉中锌蓄积水平随饲料锌添加量的增加而显著增加(P<0.05),肝胰腺中锌蓄积更明显。添加50 mg Zn/kg组(锌含量为73.25 mg Zn/kg饲料)对虾鳃组织中的Toll受体mRNA和溶菌酶mRNA表达量均显著高于未添加锌组和添加150 mg Zn/kg组(P<0.05)。添加50 mg Zn/kg组对虾肌肉、肝胰腺和血淋巴中溶菌酶活性显著高于未添加锌组(P<0.05)。添加50 mg Zn/kg组对虾肝胰腺和血淋巴中的SOD活性也显著高于未添加锌组,但与添加150 mg Zn/kg组无显著差异。而肌肉中SOD活性在添加150 mg Zn/kg组中最高。经溶藻弧菌人工急性感染后,添加50 mg Zn/kg组对虾半致死时间和全致死时间大于未添加锌组和添加150 mg Zn/kg组。本研究表明,相比摄食未添加锌组饲料和添加150 mg Zn/kg组饲料,凡纳滨对虾的免疫抗菌机能在摄取添加50 mg Zn/kg(锌含量为73.25 mg Zn/kg饲料)饲料时得到改善。
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| 关 键 词： | 凡纳滨对虾 蛋氨酸锌 Toll受体mRNA 溶菌酶mRNA 超氧化物歧化酶 溶菌酶 |
| 收稿时间： | 2010-12-13 |
| 修稿时间： | 2011-05-12 |
Effects of zinc supplementation in diet on the immunity,Vibrio resistant ability,lysozyme mRNA and Toll receptor mRNA expressions in the white shrimp(Litopenaeus vannamei) |
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| GUO Teng-fei,HUANG Xu-xiong,SU Ming,WANG Wu-gang,HUAI Xiang-jun,HU Pan,YAN Jia-qi,L Dan,HUANG Zheng-zheng. Effects of zinc supplementation in diet on the immunity,Vibrio resistant ability,lysozyme mRNA and Toll receptor mRNA expressions in the white shrimp(Litopenaeus vannamei)[J]. Journal of Fisheries of China, 2011, 35(7): 1081-1089. DOI: 10.3724/SP.J.1231.2011.17264 |
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| Authors: | GUO Teng-fei HUANG Xu-xiong SU Ming WANG Wu-gang HUAI Xiang-jun HU Pan YAN Jia-qi L Dan HUANG Zheng-zheng |
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| Affiliation: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Fisheries Technology Extension Station of Qingpu District,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Fisheries Technology Extension Station of Qingpu District,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education |
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| Abstract: | |
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| Keywords: | Litopenaeus vannamei zinc methionine Toll receptor mRNA lysozyme mRNA SOD lysozyme |
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