| 基于谷子测序开发的SSR标记多态性检测 |
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| 引用本文： | 王永芳,李伟,智慧,李海权,刁现民.基于谷子测序开发的SSR标记多态性检测[J].河北农业科学,2010,14(11):73-76. |
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| 作者姓名： | 王永芳 李伟 智慧 李海权 刁现民 |
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| 作者单位： | [1]河北省农林科学院谷子研究所,国家谷子改良中心,河北省杂粮研究实验室,河北石家庄050031 [2]中国农业科学院作物科学研究所,北京100081 |
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| 基金项目： | 国家自然科学基金重点项目，河北省自然科学基金课题，河北省农林科学院青年基金课题 |
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| 摘 要： | 利用谷子和狗尾草属其它种的24个材料,对474对基于谷子基因组测序结果设计的SSR引物进行了多态性鉴定。结果显示,有96对引物在各材料之间表现出了丰富的多态性,多态性率20.25%。本研究结果所筛选的谷子SSR标记丰富了狗尾草属遗传进行分析、种质资源研究、遗传图谱构建、目的基因定位及分子标记辅助选择可利用SSR标记的数量,同时也为谷子SSR标记核心数据库的建立奠定了基础。
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| 关 键 词： | 谷子 基因组测序 SSR引物 多态性检测 |
Selection of SSR Primers Developed from Genome Sequence of Foxtail Millet |
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| WANG Yong-fang,LI Wei,ZHI Hui,LI Hai-quan,DIAO Xian-min.Selection of SSR Primers Developed from Genome Sequence of Foxtail Millet[J].Journal of Hebei Agricultural Sciences,2010,14(11):73-76. |
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| Authors: | WANG Yong-fang LI Wei ZHI Hui LI Hai-quan DIAO Xian-min |
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| Institution: | 1.National Millet Improvement Center,Institute of Millet of Hebei Academy of Agriculture and Forestry Sciences,Cereal Crops Research Laboratory of Hebei Province,Shijiazhuang 050031,China;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | The polymorphism of 474 pairs of SSR primers designed from the genome sequence of 24 accessions of foxtail millet and closely related species in Setaria was detected.The results showed that 96 pairs of SSR primers were amplified polymorphic products in all accessions,which accounted for 20.25% of all the primers.The results of this study added more SSR markers for germplasm research,analysis of genetic diversity,genetic map construction,gene location and marker-assisted selection of Setaria,which would lay a foundation for the construction of core database of foxtail millet by SSR markers. |
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| Keywords: | Genome sequence Foxtail millet SSR primers Detection of polymorphism |
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