| 马铃薯脱毒组培苗的免疫电镜检测研究 |
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| 引用本文： | 张仲凯,李云海.马铃薯脱毒组培苗的免疫电镜检测研究[J].云南农业大学学报,1993,8(3):167-170. |
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| 作者姓名： | 张仲凯 李云海 |
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| 作者单位： | 云南省农科院生物技术研究所 昆明 |
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| 摘 要： | 应用马铃薯病毒X、Y(PVX及PVY)标准抗原和抗体,结合电子显微镜制样方法,对马铃薯脱毒组培苗进行免疫电镜检测研究.适宜工作条件室温20～25℃,抗体孵育10min、抗原吸附5min.在组培苗中检测到3种病毒粒体:PVX、PVY及类似烟草脆裂病毒(TRV)的短样杆状粒体.
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| 关 键 词： | 马铃薯脱毒组培苗 免疫电镜 检测 |
Detecting Studies on Potato Tissue Cultural Seedlings of Free-virus by Immunoeletron Microscopy |
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| ZhangZhongKai LiYunhai HeYunkung WuChengjun ZhengLiping.Detecting Studies on Potato Tissue Cultural Seedlings of Free-virus by Immunoeletron Microscopy[J].Journal of Yunnan Agricultural University,1993,8(3):167-170. |
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| Authors: | ZhangZhongKai LiYunhai HeYunkung WuChengjun ZhengLiping |
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| Institution: | Biotechnology Research Institute, Yunnan Academy of Agricutral Sciences |
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| Abstract: | Detecting research was carried on potata tissue cultural seedlings of free-virus by standard antigen and antibody of potato virus X and Y(PVX and PVY)combined with the method of making sample by electron microscope. The suitable work condition was indoor temperature, the time of hatching antibody was 10 rain and the time of adsorbed antigen was 5 min. The result of detecting is 3 kinds of virus particles in potato tissue cultural seedlings of free-virus: PVX、PVY and a kind of short-straight particle that is similar to Tobacco Rattle Virus (TRV). |
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| Keywords: | Potata Tissue Cultral seedlings of Free-virus Immunoelectron Microscopy Detecting |
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