| 绵羊Granulysin在大肠杆菌中的表达与鉴定 |
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| 引用本文： | 杨国宇,乔新安,朱彦彩,贾海丽,王月影,韩立强,王艳玲.绵羊Granulysin在大肠杆菌中的表达与鉴定[J].江西农业学报,2007,19(11):61-63. |
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| 作者姓名： | 杨国宇 乔新安 朱彦彩 贾海丽 王月影 韩立强 王艳玲 |
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| 作者单位： | 1. 河南农业大学,动物生理生化实验室,河南,郑州,450002
2. 河南科技学院,细胞工程重点实验室,河南,新乡,453003 |
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| 摘 要： | 根据GenBank中公布的绵羊Granulysin基因序列设计引物,用PCR法从重组质粒中扩增出含BamHI/XhoI酶切位点的Granulysin片段,双酶切后克隆至pGEX-4T-1载体,构建重组原核表达质粒pGEX-Granulysin,转化宿主菌大肠杆菌BL21(DE3),经IPTG诱导进行表达,SDS-PAGE鉴定,表达的融合蛋白分子量约为41 kDa,并以包含体形式存在。
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| 关 键 词： | 绵羊 颗粒溶解素 原核表达 鉴定 大肠杆菌 |
| 文章编号： | 1001-8581（2007）11-0061-03 |
| 收稿时间： | 2007-08-27 |
| 修稿时间： | 2007年8月27日 |
Expression and Identification of Ovine Granulysin in E.coli |
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| YANG Guo-yu,QIAO Xin-an,ZHU Yan-cai,JIA Hai-li,WANG Yue-ying,HAN Li-qiang,WANG Yan-ling.Expression and Identification of Ovine Granulysin in E.coli[J].Acta Agriculturae Jiangxi,2007,19(11):61-63. |
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| Authors: | YANG Guo-yu QIAO Xin-an ZHU Yan-cai JIA Hai-li WANG Yue-ying HAN Li-qiang WANG Yan-ling |
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| Abstract: | The primers were designed according to the sequences of ovine Granulysin from GenBank.A fragment of ovine Granulysin containing BamHI/XhoI was amplified from recombinant plasmid by PCR.The fragment cleaved by BamHI/XhoI was subcloned into pGEX-4T-1 vector and to construct a recombinant expression plasmid of pGEX-Granulysin.Subsequently,E.coli BL2l(DE3) competent cells were transformed by the recombinant plasmid.The fusion protein,which was induced by IPTG was identified by SDS-PAGE.The fusion protein expressed in E.coli was 41 kDa and mainly existed as inclusion bodies. |
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| Keywords: | Sheep Granulysin Prokaryotic expression Identification E coli |
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