| 通过甘蓝型油菜和白芥属间杂种后代的小孢子培养获得二体异附加系 |
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| 引用本文： | 张永泰,李爱民,陆莉,陈柳,惠飞虎,王幼平.通过甘蓝型油菜和白芥属间杂种后代的小孢子培养获得二体异附加系[J].作物学报,2006,32(11):1764-1766. |
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| 作者姓名： | 张永泰 李爱民 陆莉 陈柳 惠飞虎 王幼平 |
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| 作者单位： | 1.江苏里下河地区农业科学研究所，江苏扬州 225007；2扬州大学生物科学与技术学院，江苏扬州225009 |
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| 基金项目： | 国家自然科学基金;江苏省自然科学基金 |
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| 摘 要： | 对3株甘蓝型油菜-白芥单体异附加系（2n=39）进行小孢子培养，共获得15株单倍体植株。经GISH鉴定，其中11株n=19，4株附加有一条白芥染色体（n=20）。经秋水仙素加倍处理，获得了二体异附加系(2n=40)，这为远缘杂交后代外源基因的保存、纯合和利用提供了一条新的途径。
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| 关 键 词： | 甘蓝型油菜 白芥 小孢子培养 二体异附加系 |
| 收稿时间： | 2005-12-13 |
| 修稿时间： | 2006-04-14 |
Disomic Alien Addition Lines Generated through Microspore Culture from Progeny of Intergeneric Hybrids between Brassica napus and Sinapis alba |
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| ZHANG Yong-Tai,LI Ai-Ming,LU Li,CHEN Liu,HUI Fei-Hu,WANG You-Ping.Disomic Alien Addition Lines Generated through Microspore Culture from Progeny of Intergeneric Hybrids between Brassica napus and Sinapis alba[J].Acta Agronomica Sinica,2006,32(11):1764-1766. |
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| Authors: | ZHANG Yong-Tai LI Ai-Ming LU Li CHEN Liu HUI Fei-Hu WANG You-Ping |
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| Institution: | 1.Jiangsu Institute of Agricultural Science in the Lixiahe District, Yangzhou 225007, Jiangsu；2College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China |
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| Abstract: | Microspore culture of three monosomic alien addition lines from the hybrids between B.napus and S.alba(2n=39) was carried out.In total,fifteen microspore-derived plants were obtained,among them eleven seedlings were determined to have 19 chromosomes,and four seedlings to have 20 chromosomes.Doubled haploid plants were produced after treatment with colchicine and disomic chromosome addition lines(2n=40) were identified by GISH analysis.This method is a novel route to preserve,purify and utilize the genetic germplasm with important agricultural characteristics in further rapeseed breeding. |
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| Keywords: | Brassica napus L Sinapis alba L Microspore culture Disomic alien addition line |
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