新优彩叶植物红叶樱花外植体采集及离体培养技术研究

对红叶樱花外植体采集时期和采集部位进行研究,筛选了红叶樱花的启动、增殖和生根培养基,并研究了赤霉素和黑布遮光对株高的影响。结果表明:红叶樱花外植体的最佳采集时期在4-5月,以当年萌发的半木质化枝条中、上部芽萌发最好;启动培养基为改良MS+6-BA 1.0mg/L+NAA0.5mg/L+蔗糖30g/L+琼脂6g/L时,成芽率达到93.3%;在改良MS培养基中添加0.8mg/L的6-BA时,增殖系数达到2.21;在接种后5d进行黑布遮光处理10d,然后见光培养,可以增加株高,株高最高达2.24cm;最佳的生根培养基为1/2MS+IBA 0.05mg/L+NAA 0.05mg/L+蔗糖20g/L+琼脂6g/L,生根率达100%。

Study on Explants and Tissue Culture of Prunus serrulata Royal burgundy

Using Prunus serrulata Royal burgundy as material,in vitro culture was studied including the collecting period,bud parts,explants initial culture,subculture and rooting culture,as well as the effect of GA3 and darkness on the height of plantlet.The results showed that the germination of the upper axillary bud was optimal from the half-lignification stems in April-May,with the suitable medium MS+1.0 mg/L 6-BA+0.5 mg/L NAA+30 g/L sugar,on which the highest germination rate could reach 93.3%.The propagating ratio was 2.21 on the improved MS medium with 0.8 mg/L 6-BA.The growth rate of tissue culture seedling highly improved with 10-day darkness treatment after 5 days of inoculation,and the seedling height was 2.24 cm.The rooting rate was 100% on the medium of 1/2MS+IBA 0.05 mg/L+NAA 0.05 mg/L+sugar 20 g/L+agar 6 g/L.