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Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance |
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| Authors: | SHEN Wei-wei SONG Cheng-li CHEN Jie FU Ya-ping WU Jian-li JIANG Shao-mei |
| Affiliation: | 1.College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;2.China National Rice Research Institute, Hangzhou 310006, China;3.Jiangxi University of Finance and Economics, Nanchang 330013, China |
| Abstract: | Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia (Griseb.), a pair of specific PCR primers FZ14P1 /FZ14P2 was designed to isolate candidate disease resistance gene. The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome (TAC) library derived from Z. latifolia. A positive TAC clone (ZR1) was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar... |
| Keywords: | Zizania latifolia transformation-competent artificial chromosome library resistance-gene analog Oryza sativa bacterial blight resistance gene transfer |
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