| 黑木耳漆酶酶学性质的研究 |
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| 引用本文： | 肖楚 刘佳 许修宏. 黑木耳漆酶酶学性质的研究[J]. 中国农学通报, 2011, 27(25): 158-161 |
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| 作者姓名： | 肖楚 刘佳 许修宏 |
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| 作者单位： | 东北农业大学,哈尔滨,150030 |
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| 基金项目： | 科技部十一五科技支撑项目"野生食用菌资源保育与利用技术研究"，黑龙江省科技厅计划项目"黑龙江省农业微生物菌种资源共享平台建设" |
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| 摘 要： | 本实验从黑木耳液体发酵液中提取漆酶，对其酶学性质进行研究，为该漆酶的应用提供有价值的理论数据。对漆酶发酵液进行硫酸铵分级沉淀和透析，制得粗酶液。采用分光光度法，以ABTS[2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]为底物测定漆酶活力。结果表明：黑木耳漆酶在55℃以下保温，漆酶活力和热稳定性良好。最适温度为45℃。温度超过65℃，出现明显的抑制作用。黑木耳漆酶最适pH为4.0，pH高于6.5，出现明显的抑制作用。金属离子对酶活表现出促进或抑制的作用。
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| 关 键 词： | 畜牧业 畜牧业 低碳经济 污染 资源 节粮 |
| 收稿时间： | 2011-05-04 |
| 修稿时间： | 2011-06-17 |
Studies on Enzymatic Properties of Laccase from auricularia |
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| Xiao Chu,Liu Jia,Xu Xiuhong. Studies on Enzymatic Properties of Laccase from auricularia[J]. Chinese Agricultural Science Bulletin, 2011, 27(25): 158-161 |
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| Authors: | Xiao Chu Liu Jia Xu Xiuhong |
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| Affiliation: | Xiao Chu,Liu Jia,Xu Xiuhong (Northeast Agriculture University,Harbin 150030) |
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| Abstract: | Collecting laccase from the fermentation liquid of auricularia and studying the enzymatic properties in the experiment can provide worthful theory data for application of laccase.Laccase liquid is prepared by （NH 4） 2 SO 4 fractional precipitation and dialysis.By using spectrophotometry and choosing ABTS [2,2 ＇ -Azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）],the activity of laccase can be determined.The results showed that when the laccase was insulated below 55℃,the relative activity and thermal stability was good.The optimal temperature was 45℃.It could be inhibited when the temperature was above 65℃.The optimal pH was 4.0.It could be inhibited when the pH was above 6.5.Metal ions could inhibit or enhance the activity of laccase. |
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| Keywords: | Auricularia laccase enzymatic properties |
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