特晚熟红桔JZT(P)-1胚珠败育的胚胎学研究

为进一步探明晚熟无核红桔的无核机理，为晚熟柑橘种质资源的开发利用提供可靠的科学依据，以特晚熟无核红桔JZT(P)-1为实验材料，采用直接解剖、连续石蜡切片、显微技术等方法重点对其胚珠败育过程进行系统的观察研究。结果表明，JZT(P)-1胚珠中的孢原细胞、胚囊原始细胞以及不同发育程度的胚囊基本上都败育，极少数胚珠发育成为健康的种子。在幼果前50天的发育中，胚囊原始细胞多起源发育，发生时间紊乱，使得珠心中早期孢原细胞及不同发育期胚囊的正常发育受到干扰，促使胚珠中各雌性结构的败育。幼果中雌性败育率极高，通过石蜡切片观察到正常胚的发育十分困难。基本成熟的果中，种子数0~4粒，平均种子数(1.3±0.42)粒，经济无核率高达100%。正常种子多胚。研究结果为探明特晚熟红桔新种质JZT(P)-1的无核机理提供了胚胎学证据。

Embryological Research on the Ovule Abortion Development of Super Late-maturing Cultivar JZT(P)-1 Tangerine

To further prove the seedless mechanism on JZT(P)-1 and provide reliable scientific evidence to the exploitation of late-maturing Citrus germplasm,the ovule abortion process of Citrus reticulata,named JZT(P)-1,was systematically studied by a series of direct-dissection,consecutive paraffin sections and microscopic technique.It was found that the archesporial cell,embryo sac primitive cells and embryo sacs in different developing period were almost abortive.Only a few of seeds could develop into health seeds...