| 龙眼SRAP反应体系的建立和优化 |
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| 引用本文： | 赵玉辉 郭印山 傅嘉欣 周佳 黄穗生 刘成明. 龙眼SRAP反应体系的建立和优化[J]. 中国农学通报, 2009, 25(18): 409-412 |
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| 作者姓名： | 赵玉辉 郭印山 傅嘉欣 周佳 黄穗生 刘成明 |
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| 作者单位： | 华南农业大学园艺学院,广州,510642 |
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| 基金项目： | 国家自然科学基金项目"荔枝童期、成熟期性状的QTL定位"，中国博士后科学基金项目"龙眼重要性状的基因定位研究" |
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| 摘 要： | 采用分步优化的方法对影响龙眼SRAP-PCR反应的模板DNA用量、Mg2+浓度、dNTP浓度、引物浓度、TagDNA聚合酶用量等进行了研究。确立了适合龙眼SRAP分析的反应体系，即体系总体积25μl，包含1×PCR Buffer ，Mg2+ 2.0mmol/L，dNTPs 0.5 mmol/L，引物0.3μmol/L，模板DNA 10ng， TaqDNA聚合酶1.5 U。结果表明,该体系能很好地满足龙眼基因组SRAP扩增的要求，SRAP标记应用于龙眼遗传研究是可行的。
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| 关 键 词： | 早香橘橙 早香橘橙 套袋 时间 色泽 |
| 收稿时间： | 2009-04-15 |
| 修稿时间： | 2009-05-06 |
Establishment and Optimization of SRAP Reaction System in Longan |
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| Zhao Yuhui,Guo Yinshan,Fu Jiaxin,Zhou Jia,Huang Suisheng,Liu Chengming. Establishment and Optimization of SRAP Reaction System in Longan[J]. Chinese Agricultural Science Bulletin, 2009, 25(18): 409-412 |
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| Authors: | Zhao Yuhui Guo Yinshan Fu Jiaxin Zhou Jia Huang Suisheng Liu Chengming |
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| Affiliation: | (College of Horticulture, South China Agricultural University, Guangzhou 510642) |
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| Abstract: | To establish appropriate longan SRAP-PCR reaction system, factors that affected the amplified result of SRAP such as amounts of DNA and Taq DNA polymerase, concentrations of Mg2+, dNTP and primer were studied by staged optimization method. Reaction system suitable for SRAP analysis was established. The reaction mixture (25μl) contained, 1 ×PCR Buffer, 2.0mmol/L Mg2+, 0.5 mmol/L dNTPs, 0.3μmol/L primer, 10 ng DNA template, and 1.5 U Taq DNA polymerase. The results showed the reaction system could meet SRAP amplification requirement of longan genome, and SRAP marker for longan genetic study was feasible. |
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| Keywords: | SRAP |
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