| C型口蹄疫病毒VPI基因的克隆及真核表达载体的构建 |
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| 引用本文： | 杨生海,殷宏,张杰,刘永生,张继乐,曾巧英,陈豪泰,马丽娜,马艳平,丁耀忠.C型口蹄疫病毒VPI基因的克隆及真核表达载体的构建[J].安徽农业科学,2009,37(10):4416-4419. |
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| 作者姓名： | 杨生海 殷宏 张杰 刘永生 张继乐 曾巧英 陈豪泰 马丽娜 马艳平 丁耀忠 |
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| 作者单位： | 甘肃农业大学动物医学院,甘肃兰州,730070;中国农业科学院兰州兽医研究所畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州,730046;中国农业科学院兰州兽医研究所畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州,730046;甘肃农业大学动物医学院,甘肃兰州,730070 |
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| 基金项目： | 甘肃省科技重大专项项目(0801NKDA034); 兰州市科技计划项目(2008-1-167) |
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| 摘 要： | 应用PT-PCR扩增C型口蹄疫病毒（FMDV）VP1基因,将其克隆到pMD18-T载体中,并对克隆载体和VP1基因进行序列分析。将VP1基因和选择标记基因——二氢叶酸还原酶（dhfr）基因插入真核表达载体pCI-neo中,对所构建的重组真核表达载体进行PCR扩增、酶切鉴定和序列分析。结果表明,VP1基因与GenBank中标准毒株（登录号EU553893）VP1的序列同源性为92.8%,VP1基因的真核表达载体pCI-VP1-D构建成功。
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| 关 键 词： | C型口蹄疫病毒 VPI基因 真核表达载体 |
Cloning and Construction of Eukaryotic Expression Vector of VP1 Gene from Foot-and-mouth Disease Virus of Type C |
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| YANG Sheng-hai et al.Cloning and Construction of Eukaryotic Expression Vector of VP1 Gene from Foot-and-mouth Disease Virus of Type C[J].Journal of Anhui Agricultural Sciences,2009,37(10):4416-4419. |
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| Authors: | YANG Sheng-hai |
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| Institution: | YANG Sheng-hai et al(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu 730070) |
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| Abstract: | The VP1 gene from foot-and-mouth disease virus of type C was amplified by RT-PCR,and it was cloned into pMD18-T vector.The sequences of the cloning vector and VP1 gene were analyzed.The VP1 gene and selectable marker gene dihydrofolate reductase(dhfr) were inserted into eukaryotic expression vector pCI-neo,and the constructed recombinant eukaryotic expression vector was amplified by PCR,and it was analyzed by enzyme digestion and sequence analysis.The results showed that The sequence homology of VP1 gene an... |
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| Keywords: | Foot-and-mouth disease virus of type C VP1 gene Eukaryotic expression vector |
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