| 关中驴线粒体DNA D-loop多态性分析 |
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| 引用本文： | 雷初朝,陈宏,王德解,杨公社,孙维斌. 关中驴线粒体DNA D-loop多态性分析[J]. 中国畜牧杂志, 2004, 40(4): 10-12 |
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| 作者姓名： | 雷初朝 陈宏 王德解 杨公社 孙维斌 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100
2. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;徐州师范大学生物技术研究所,江苏,徐州,221009 |
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| 基金项目： | 教育部留学回国人员科研启动基金，西北农林科技大学校科研和教改项目 |
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| 摘 要： | 本文对 6头关中驴线粒体DNAD loop区 399bp的核苷酸序列进行了分析。结果发现 ,关中驴的D loop区核苷酸变异只有转换 1种形式。 6头关中驴D loop区的核苷酸序列组成 3种单倍型 ,单倍型比例为 5 0 .0 0 % ,说明关中驴mtDNA遗传多样性正逐步丧失 ,需要加强驴种质资源保护。以欧洲驴D loop序列为对照 ,关中驴 3种单倍型的核苷酸变异率分别为 4 .2 1%、4 .5 1%和 0 .2 5 %。在获得的 399bpD loop碱基序列中 ,共检测出核苷酸多态位点18个 ,多态位点比例为 4 .5 1%。从D loop区核苷酸序列的 3种单倍型分析 ,发现关中驴可能有 2种不同的母系起源
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| 关 键 词： | 畜牧、兽医科学基础学科 线粒体DNA Dloop 多态性 关中驴 |
| 文章编号： | 0258-7003(2004)04-0010-03 |
| 修稿时间： | 2003-02-17 |
Analysis on Polymorphism of mtDNA D-loop Region in Guanzhong Donkey |
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| LEI Chu-zhao,CHEN Hong. Analysis on Polymorphism of mtDNA D-loop Region in Guanzhong Donkey[J]. Chinese Journal of Animal Science, 2004, 40(4): 10-12 |
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| Authors: | LEI Chu-zhao CHEN Hong |
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| Affiliation: | LEI Chu-zhao~1,CHEN Hong~ |
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| Abstract: | The mitochondrial DNA D-loop region of 6 Guanzhong donkey samples were analyzed in this study. The size of the D-loop region was about 399 bp. The results showed that all mutations came from nucleotide transition. Comparisons of these 6 partial D-loop sequences revealed 3 mitochondrial haplotypes in Guanzhong donkey, the haplotype rate was (50.00%,) demonstrating that the mitochondrial genetic diversity is deprivating. It is imperious to strength protecting the donkey resources. The nucleotide mutational rate of 3 haplotypes of No.1, No.3 and No.4 was 4.26%, 4.51% and (0.25%) respectively. A total of 18 variation sites were detected and the mutation rate was 4.51% in 6 donkey samples. After analyzing the mtDNA D-loop 3 haplotypes, it was possible that Guanzhong donkey has two maternal origins. |
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| Keywords: | D-loop |
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