纳他霉素对灰葡萄孢不同生育阶段菌体的毒力及其生物学性状的影响

以多菌灵为对照药剂,采用生长速率法、悬滴法和浸渍法分别测定了不同温度下纳他霉素对灰葡萄孢Botrytis cinerea菌丝生长、分生孢子萌发和菌核萌发的毒力,研究了常温下两种药剂对菌株产孢和菌核形成的影响。结果表明:纳他霉素及多菌灵对灰葡萄孢不同生育阶段菌体的抑制作用随温度降低而有不同程度增强;分生孢子对药剂最为敏感;温度对药剂对菌丝毒力的影响最显著;多菌灵对菌株不同发育阶段的抑制活性均高于纳他霉素。供试药剂对菌株产孢时间和菌核产生时间无显著影响,但多菌灵可显著刺激菌株产孢和菌核形成。

Toxicity of Natamycin to Botrytis cinerea at Different Growth Stages and its Effect on BiologicalCharacteristics

Toxicity of natamycin on the mycelial growth,conidial germination and sclerotium germination of Botrytis cinerea was tested,using carbendazim as control,through growth rate method,hanging drop slide method and pickling method,respectively.The effects of the two fungicides on the sporulation and sclerotigenic of Botrytis cinerea were also studied.The results indicated that toxicity of natamycin and carbendazim to Botrytis cinerea at different growth stages enhanced as the temperature decreased.Conidia was the most sensitive to the two fungicides.Temperature showed the most significant effect on mycelial growth.The activity of carbendazim was higher than that of natamycin.The two fungicides have no effect on the time of sporulation and sclerotigenic of Botrytis cinerea,but carbendazim can stimulate the producing of sporulation and sclerotigenic significantly.