| 稻平脐蠕孢的分离、鉴定及对作物的安全性 |
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| 引用本文： | 耿锐梅,张建萍,余柳青. 稻平脐蠕孢的分离、鉴定及对作物的安全性[J]. 浙江农业学报, 2008, 20(6): 0 |
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| 作者姓名： | 耿锐梅 张建萍 余柳青 |
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| 作者单位： | 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州,310006 |
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| 基金项目： | 国家863 ， 浙江省自然科学基金 ， 中央级公益性科研院所专项资金 |
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| 摘 要： | 为了筛选获得可用于防治李氏禾的病原菌菌株,采用孢子形态观察和ITS1-5.8S-ITS2序列分析法对分离自云南西双版纳稻田感病李氏禾植株上的两株病原真菌菌株进行了鉴定,采用离体叶片生测法评价其除草活性,采用盆栽生测法评价其对主要作物的安全性.结果表明,两株病原真菌分别被鉴定为稻平脐蠕孢(Bipolaris oryzae)和稻平脐蠕孢有性型[Bipolaris oryzae (teleomorph:Cochliobolus miyabeanus)].稻平脐蠕孢发酵液对李氏禾的致病性最高为88.9;稻平脐蠕孢有性型发酵液配制的菌悬液对李氏禾的致病性最高为100.两个菌株对水稻、玉米、黄瓜及番茄等主要农作物安全.
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| 关 键 词： | 稻平脐孺孢 稻平脐孺孢有性型 分子鉴定 李氏禾 生物防治 作物安全性 |
Isolation,identification and herbicidal activity of Bipolaris sp. and its safety on crops |
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| GENG Rui-mei,ZHANG Jian-ping,YU Liu-qing. Isolation,identification and herbicidal activity of Bipolaris sp. and its safety on crops[J]. Acta Agriculturae Zhejiangensis, 2008, 20(6): 0 |
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| Authors: | GENG Rui-mei ZHANG Jian-ping YU Liu-qing |
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| Affiliation: | GENG Rui-mei,ZHANG Jian-ping,YU Liu-qing(State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China) |
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| Abstract: | In order to screen pathogen strains for biological control of the weed,Leersia hexandra Swartz,identification of two strains isolated from susceptible L.hexandra on rice field in Xishuangbanna district of Yunnan province was conducted by the spore morphology observation and sequence analysis of their internal transcribed spacers(ITS1-5.8S-ITS2).The herbicidal activity of the fungi against L.hexandra was evaluated by detached leaf bioassay,and the safety of crop was tested using pot bioassay method.The resul... |
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| Keywords: | Bipolaris oryzae Bipolaris oryzae(teleomorph:Cochliobolus miyabeanus) molecular identification Leersia hexandra biological control crop safety |
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