| 谷氨酰胺和IGF-I对晋岚绒山羊卵母细胞体外成熟的影响 |
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| 引用本文： | 张春香,秦雪,郑亚琳,张彩霞,岳文斌,任有蛇,靳黎.谷氨酰胺和IGF-I对晋岚绒山羊卵母细胞体外成熟的影响[J].中国养羊,2014(5):26-28. |
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| 作者姓名： | 张春香 秦雪 郑亚琳 张彩霞 岳文斌 任有蛇 靳黎 |
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| 作者单位： | 山西农业大学动物科技学院,太谷030801 |
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| 基金项目： | 山西省科技攻关计划项目（20110311029） |
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| 摘 要： | 为了探讨在成熟培养液中添加谷氨酰胺和IGF-I对晋岚绒山羊母羔卵母细胞体外成熟的影响。随机选择12只6-8周龄晋岚绒山羊母羔为试验动物，用FSH进行超数排卵，活体采集卵母细胞，并对未成熟的卵母细胞进行体外成熟培养。在培养液中添加0、50、100和150μg/mL的谷氨酰胺及0、25、50和100 ng/mL的IGF-I。结果表明：培养液中谷氨酰胺添加量为100μg/mL时，能显著提高卵母细胞成熟率（P〈0.05）；在培养液中添加100μg/mL谷氨酰胺的基础上，再添加50 ng/mL的IGF-I可以进一步显著提高卵母细胞的成熟率（P〈0.01）。
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| 关 键 词： | 晋岚绒山羊母羔 卵母细胞 体外成熟 谷氨酰胺 IGF-I |
Effect of Glutamine and IGF-I on the Oocyte maturation of Jinlan Cashmere Goats |
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| Institution: | Zhang Chun-xiang, Qin Xue, Ren You-she, et al (Animal Science College,Shanxi Agricultural University,Taigu 030801 ,China) |
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| Abstract: | The objective of the study was to investigate the effect of glutamine and IGF-I on oocyte maturation in Jinlan Cashmere goats. Twelve 6-8 week-old Jinlan cashmere female kids were selected to superovulate by FSH injection. The immature oocytes were collected by ovum pick-up, and culture in vitro maturation with supplementation of different concentration of glutamine （0,50,100 and 150μg/mL）and IGF-I （0,25,50 and 100 ng/mL）in maturation media. The results showed that the maturation rate of oocytes could be significantly improved by adding 100μg/mL glutamine to the maturation media （P〈0.05）. After addition 100μg/mL glutamine,adding 50 ng/mL IGF-I could markedly increase the rate of occytes maturation（P〈0.01）. |
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| Keywords: | IGF-I Jinlan cashmere female kids oocyte in vitro maturation glutamine IGF-I |
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