我国部分地区牛羊弓形虫血清流行病学调查 |
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引用本文: | 周昕薛,;周欢,;宁晓冬,;李静,;菅复春,;张龙现,;赵青玉,;宁长申.我国部分地区牛羊弓形虫血清流行病学调查[J].中国养羊,2014(5):43-46. |
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作者姓名: | 周昕薛 ;周欢 ;宁晓冬 ;李静 ;菅复春 ;张龙现 ;赵青玉 ;宁长申 |
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作者单位: | [1]河南农业大学牧医工程学院,郑州450002; [2]河南省人兽共患病国际联合实验室,郑州450002 |
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基金项目: | 国家现代肉羊产业技术体系(nyeytx-39) |
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摘 要: | 为了解我国牛羊弓形虫病流行情况,应用间接血凝试验(IHA)对河南、山东、山西、内蒙古、云南、贵州6省区151份牛血清、50份奶样、490份羊血清进行了弓形虫病血清流行病学调查。结果显示:151份被检牛血清和50份牛奶样品,弓形虫抗体均为阴性。490份羊血清弓形虫抗体总阳性率5.71%,其中母羊、公羊血清阳性率分别为4.03%和9.79%;山羊、绵羊、杂交羊血清阳性率分别为6.58%、4.81%和5.13%;阳性率最高的为公山羊(13.2%),最低的为母绵羊(2.96%)。28份阳性羊血清中,75%的抗体滴度为1:64,25%的抗体滴度为1:256。1岁后的羊,随年龄增长,血清阳性率升高。
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关 键 词: | 牛 羊 弓形虫 血清抗体阳性率 间接血凝试验 |
Seroprevalence of Toxoplasma Gondii in Cattle,Sheep and Goats in Partial Area of China |
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Institution: | Zhou Xin-xue, Zhou Huan, Jian Fu-chun, et al ( 1.College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; 2.International Joint Research Laboratory for Zoonotic Disease ofHenan, Henan Agricultural University, Zhengzhou 450002, China) |
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Abstract: | The objectives of this study were to determine the seroprevalence of Toxoplasma gondii in cattle,sheep and goat.151 serum samples from cattle,50 milk samples from cows,490 serum samples from sheep and goats were collected from Henan, Shandong,Shanxi,Neimenggu,Yunnan,Guizhou provinces. These samples’anti-T.gondii antibodies were examined using a com-mercial indirect hemagglutination assay kits. The result showed that specific IgG against T. Gondii of 151 samples from cattle were all negative;while in 490 samples from sheep and goats,the overall seroprevalence rate was 5.71%, the female and the male had different seroprevalences surprisingly(4.03%and 9.79%respectively);the goats,sheep,mixed-breed had comparable seropreva-lences(6.58%,4.81%and 5.13%,respectively),the male goats had the highest seroprevalence of T. gondii(13.2%)and the fe-male sheep had the lowest one(2.96%).In 28 positive samples,75%serum antibody titer was 1:64 and the 25%was 1:256. Sero-prevalence of T. gondii infection increased significantly with the age of the assessed sheep or goats after 1-year-old. |
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Keywords: | sheep cattle Toxoplasma gondii seroprevalence IHA |
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