Quantification and distribution of kafirins in the kernels of sorghum cultivars varying in endosperm hardness |
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| Institution: | 1. Facultad de Estudios Superiores Cuatitlán, Universidad Nacional Autónoma de México, Cuautitlán, 54714, Mexico;2. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, 76230, Mexico;3. Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ajuchitlán, 76280, Mexico;1. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia;2. The Institute of Molecular Bioscience, The University of Queensland, Brisbane, QLD, 4072, Australia;3. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia;4. Department of Food Science, Purdue University, 745 Agricultural Mall Drive, West Lafayette, IN, 47907-2009, USA;1. Laboratorio de Calidad de Cultivos para Uso Humano y Pecuario, Campo Experimental Centro Altos de Jalisco, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Av. Biodiversidad 2470, CP47600, Tepatitlán de Morelos, Jalisco, Mexico;2. Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), CP, 04510, Ciudad de, Mexico;3. Laboratorio de Calidad de Maíz. Campo Experimental Valle de México, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Km. 13.5 Carretera Los Reyes-Texcoco, Coatlinchan, Mpio, Edo. de, C.P., 56250, Texcoco, Mexico;4. Colegio de Postgraduados en Ciencias Agrícolas, Km 36.5, Carretera México-Texcoco, 56230, Montecillo, Texcoco, Mexico |
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| Abstract: | Kafirins extracted from the endosperm of seven sorghum Sorghum bicolor (L.) Moench] cultivars were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). Kafirins extracted from the vitreous and floury endosperm portions within the kernel were also analysed by these techniques. The ELISA results indicated that the level of all the three kafirins was high in the hard endosperm kernels. The level of γ-kafirin was particularly higher in the vitreous endosperm portions of these kernels. The ratio of γ-kafirin to the α-kafirin was, however, higher for the floury portions of soft kernels. Tissue print immunoblotting revealed that the β- and γ-kafirins were concentrated in the central floury endosperm portions of soft kernels, whilst α-kafirin was distributed more uniformly throughout the endosperm. In contrast, all three kafirins were distributed uniformly throughout the endosperms of hard kernels. The data indicate that the content, as well as the distribution, of kafirins within the kernel is different in grains varying in endosperm hardness. |
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