进境澳大利亚大麦夹杂油菜茎基溃疡病菌的检疫鉴定

采用常规平板分离法,从进境澳大利亚大麦中夹杂的油菜籽上获得1株疑似油菜茎基溃疡病菌的菌株01829.通过致病性测定、形态学观察、特异性引物扩增、ITS序列比对分析,对01829进行了种类鉴定.结果表明:菌株01829在PDA培养基上生长较慢,菌落边缘不整齐,产生大量分生孢子器和分生孢子;采用特异性引物对LMR1-D和L...

Quarantine identification of Leptosphaeria maculans on Hordeum vulgare imported from Australia

The fungal strain 01829 was isolated from the oilseed rape mixed in Hordeum vulgare imported from Australia by using traditional tissue separation method. Morphological observation, specific PCR detection, ITS sequence analysis and pathogenicity test were carried out to identify the strain 01829. The morphological results showed that the isolate 01829 grew slowly with irregular colony edges on PDA and produced abundant pycnidia and conidia. In specific PCR tests, the primer pairs LMR1-D and Lmb amplified a positive fragment from the DNA of isolate 01829. Its ITS sequence was sequenced. Analysis of the sequence with the related sequences retrieved from GenBank showed that the strain 01829 clustered into Leptosphaeria maculans clade. The pathogenicity test showed that the strain 01829 could cause leaf spot of cotyledons and canker lesion of stem. Based on these results, the isolate 01829 was identified as L.maculans. To our knowledge, this is the first interception report of L.maculans mixed into H.vulgare imported from Australia to China.