凤梨亚科光萼荷属与其近源属亲缘关系的ISSR分子鉴定

选用12对ISSR引物对14个从属于凤梨亚科中光萼荷属(Aechmea)与近源属的物种进行聚类分析。14个物种被分为3个家族,其中一个是由11个物种组成的大家族,记为A和其他3个光萼荷亚属分别组成的3个小家族。ISSR分子标记的分类情况表明,光萼荷属的紫红光萼荷(Aechmea rubrolilacina),红苞光萼荷(Aechmea bracteata),绿叶光萼荷(Aechmea chlorophylla)和芬德光萼荷(Aechmea fendleri)为多起源;而光叶凤梨(Aechmea ramosa var. festiva),美叶光萼荷(Aechmea fasciata)和泰氏光萼荷(Aechmea tessmanii)为单起源,说明光萼荷属物种的复杂性与多样性。在与近源属的亲缘关系分析中,光萼荷亚属的紫红光萼荷(Aechmea rubrolilacina)和红苞光萼荷(Aechmea bracteata)与翼办美花凤梨(Portea alatisepala)和具毛赫氏凤梨(Hobenbergia lanata)亲缘关系很近;而肉穗凤梨亚属(Macrochordion)则与卜氏拟心花凤梨(Canistropsis burchellii)亲缘关系较近。本实验对光萼荷属是否来源于近源属的杂交以及凤梨亚科的分类标准科学化进行了研究。

Moleculer Identification of Aechrmea and Relative Genera in Bromeliaceae. By ISSR

12 Inter Simple Sequence Repeat(ISSR)primers were used to detect genetic variation among 4 genera,including 14 individuals of Bromeliaceae.Percentage of polymorphic bands(PPB)among different species was evident high about 97%.According to deodrogram,14 species were divided into four provenance areas,among which Aechmea rubroliifolia,Aechmea bracteata,Aechmea chlorophylla and Aechmea fendleri are morphological;meanwhile Aechmea ramosa var.festiva,Aechmea fasciata and Aechmea tessmanii are monophyletic.These results show that Aechmea exhibits high genetic diversity and complexity.According to the relationship between subgenera of Aechmea and relative genera,Aechmea subgenera had high relationship with Hobenbergia lanata and subgenera of Macrochordion(Aechmea chlorophylla)withCanistropsis burchellii.Detail information and illustration about origin for Aechmea and standard taxa for Bromeliaceae were discussed in this study.