| 腰果角盲蝽气味结合蛋白基因HtheOBP3的克隆及组织表达分析 |
| |
| 引用本文： | 张绍华,孟倩倩,刘爱勤,颜日辉,林先武,孙世伟,李付鹏,王政. 腰果角盲蝽气味结合蛋白基因HtheOBP3的克隆及组织表达分析[J]. 植物保护学报, 2023, 50(1): 71-80 |
| |
| 作者姓名： | 张绍华 孟倩倩 刘爱勤 颜日辉 林先武 孙世伟 李付鹏 王政 |
| |
| 作者单位： | 中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533;海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228;中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533;海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228;重庆市疾病预防控制中心消毒与媒介生物控制所, 重庆 400042 |
| |
| 基金项目： | 国家重点研发计划(2020YFD1001202);海南省自然科学基金面上项目(319MS087);中国热带农业科学院基本科研业务费专项(1630142018016) |
| |
| 摘 要： | 为明确腰果角盲蝽Helopeltis theivora气味结合蛋白3(odorant binding protein 3,OBP3)功能及其嗅觉感受机制，利用PCR技术结合c DNA末端快速扩增（rapid amplification of c DNA ends,RACE）技术克隆其cDNA全长序列，利用多个生物信息学软件对其进行序列分析，并通过实时荧光定量PCR(real-time quantitative PCR,qPCR)技术检测其在腰果角盲蝽成虫不同组织中的表达量。结果显示，腰果角盲蝽HtheOBP3基因（GenBank登录号为QHI06949）开放阅读框为474 bp，编码157个氨基酸残基，预测蛋白分子量约为17.15 kD，等电点为5.14，无信号肽和跨膜结构，蛋白氨基酸序列中具有6个保守半胱氨酸残基和性信息素结合蛋白-普通气味结合蛋白（pheromone binding protein-general odorant binding protein,PBP-GOBP）家族的保守结构域。HtheOBP3蛋白具有6个α-螺旋和3对二硫键，其中5个α-螺旋形成1个结合口袋。腰...
|
| 关 键 词： | 腰果角盲蝽 气味结合蛋白 基因克隆 序列分析 组织表达 |
| 收稿时间： | 2021-08-28 |
Cloning and tissue expression profiling of the odorant binding protein gene HtheOBP3 in tea mosquito bug Helopeltis theivora |
| |
| ZHANG Shao-hu,MENG Qian-qian,LIU Ai-qin,YAN Ri-hui,LIN Xian-wu,SUN Shi-wei,LI Fu-peng,WANG Zheng. Cloning and tissue expression profiling of the odorant binding protein gene HtheOBP3 in tea mosquito bug Helopeltis theivora[J]. Acta Phytophylacica Sinica, 2023, 50(1): 71-80 |
| |
| Authors: | ZHANG Shao-hu MENG Qian-qian LIU Ai-qin YAN Ri-hui LIN Xian-wu SUN Shi-wei LI Fu-peng WANG Zheng |
| |
| Affiliation: | Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China;Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China; Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China;Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China;Department of Disinfection and Vector Control, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China |
| |
| Abstract: | |
| |
| Keywords: | Helopeltis theivora odorant binding protein gene cloning sequence analysis tissue expression profiling |
|
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
|
点击此处可从《植物保护学报》下载免费的PDF全文 |
|
