天津市烟粉虱隐种鉴定及其携带TYLCV、内共生菌情况和抗药性监测

为明确天津市烟粉虱Bemisia tabaci隐种的类别及其寄主适应性、传毒能力、携带内共生菌情况和抗药性，采用mt COI酶切法对从武清、西青、蓟州和宁河4个区的番茄、黄瓜及辣椒3种寄主上采集的12个烟粉虱种群进行隐种鉴定，采用PCR检测其携带番茄黄化曲叶病毒（tomato yellow leaf curl virus,TYLCV）和内共生菌情况，并采用浸叶法测定其对4种常用药剂的抗性。结果表明，采集的烟粉虱种群以MED隐种为主，占所有检测个体的93.33%，有3个种群为MED和MEAM1隐种混合发生。所有检测个体中有36.25%的个体携带TYLCV，在6个种群中检测到TYLCV，其中5个种群有超过50%的个体携带TYLCV。在12个种群中共检测到Hamiltonella、立克次氏体Rickettsia、Cardinium和杀雄菌属Arsenophnus共4种内共生菌，携带个体比例分别为90.63%、48.96%、43.75%和8.33%，进一步对内共生菌协同感染情况进行分析，发现有HARC、HRC、HAC、HR、HC和AC共6个协同感染型，感染率分别为4.17%、28.13%、3....

Cryptic Bemisia tabaci species harboring tomato yellow leaf curl virus and endosymbionts and insecticide resistance monitoring in Tianjin

To define the cryptic species of tobacco whitefly Bemisia tabaci, as well as their host fitness,virus transmission, facultative symbiont vectoring and insecticide resistance in Tianjin, the mitochondrial cytochrome oxidase I(mtCOI) gene sequences was used to identify 12 populations of B. tabaci collected from three host plants(tomato, cucumber and pepper) in four districts(Wuqing, Xiqing, Jizhou and Ninghe) in Tianjin. Tomato yellow leaf curl virus(TYLCV) and endosymbionts were detected in these populations by PCR. The resistance to four insecticides was also monitored in the 12 B. tabaci populations. The results indicated that the Mediterranean(MED) species accounted for 93.33% among the total whitefly samples tested, and there were three mixed B. tabaci populations with MED and Middle East-Asia Minor 1(MEAM1). The rate of TYLCV-carrying whiteflies was 36.25% of the total tested samples, and TYLCV was detected in six populations, five of which had a virus-carrying rate of more than 50%. The endosymbionts Hamiltonella, Rickettsia, Cardinium and Arsenophonus of B. tabaci were detected in 12 populations, accounting for 90.63%, 48.96%, 43.75% and 8.33%, respectively. Further analysis of the co-infection of the four endosymbionts indicated that there were six co-infection types, including HARC, HRC, HAC, HR, HC and AC, and the infection rates were 4.17%, 28.13%,3.13%, 14.58%, 5.21% and 1.04%, respectively. Generally, B. tabaci in Tianjin was more sensitive to abamectin(LC₅₀: 0.05-0.66 mg/L), but more resistant to thiamethoxam(LC₅₀: 267.63-2 998.80 mg/L).The toxicity of the four insecticides to nine populations was ranked as followed: avermectin>cyantraniliprole>imidacloprid>thiamethoxam, and to the other three populations, avermectin>imidacloprid>cyantraniliprole>thiamethoxam.