| 大鲵糖肽组分的高效液相色谱分析及其抗氧化活性研究 |
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| 引用本文： | 金桥,魏芳,佟长青,李伟,孔亮.大鲵糖肽组分的高效液相色谱分析及其抗氧化活性研究[J].北京农学院学报,2011,26(1):27-29. |
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| 作者姓名： | 金桥 魏芳 佟长青 李伟 孔亮 |
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| 作者单位： | 金桥,魏芳,佟长青,JIN Qiao,WEI Fang,TONG Chang-qing(辽宁省水产品加工及综合利用重点开放实验室,大连海洋大学食品工程学院,辽宁,大连,116023);李伟,LI Wei(辽宁省水产品加工及综合利用重点开放实验室,大连海洋大学食品工程学院,辽宁,大连,116023;张家界(中国)金驰大鲵生物科技有限公司,湖南,张家界,427400);孔亮,KONG Liang(大连海洋大学海洋环境工程学院,辽宁,大连,116023) |
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| 基金项目： | 大连市科技局项目，辽宁省教育厅2009度高等学校科研项目计划 |
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| 摘 要： | 利用高效液相色谱方法对大鲵糖肽进行分析,使用Sino Chrom C18反相柱和紫外可变波长检测器,并测定大鲵糖肽的羟基自由基清除能力。结果表明：大鲵糖肽组分的最佳液相色谱检测条件为：检测波长280 nm;流动相为V（乙腈）∶V（甲醇）∶V（0.1%三氟乙酸）=5∶5∶90;流速1.0 mL/min;进样量20μL。大鲵糖肽在5.78μg/mL浓度时,羟基自由基的清除率达60.19%,具有较好的抗氧化活性。
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| 关 键 词： | 大鲵 糖肽 高效液相色谱 抗氧化活性 |
HPLC analysis and antioxidative activity of glycopeptides from Andrias davidianus |
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| JIN Qiao,WEI Fang,TONG Chang-qing,LI Wei,KONG Liang.HPLC analysis and antioxidative activity of glycopeptides from Andrias davidianus[J].Journal of Beijing Agricultural College,2011,26(1):27-29. |
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| Authors: | JIN Qiao WEI Fang TONG Chang-qing LI Wei KONG Liang |
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| Institution: | JIN Qiao1,WEI Fang1,TONG Chang-qing1,LI Wei1,2,KONG Liang3(1.Key and Open Laboratory of Aquatic Products Processing and Utilization of Liaoning Province,Dalian Ocean University,Dalian Liaoning 116023,China,2.Jinchi Giant Salamander Biological Technology Co.,Ltd,Zhangjiajie(China),Zhangjiajie,Hunan 427400,3.College of Marine Environmental Engineering,China) |
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| Abstract: | Glycopeptides from Andrias davidianus(ADGP) were analysed by HPLC.C18 column Sino Chrom(5 μm,4.6 mm×150 mm) and ultraviolet detector were used.The effect of ADGP on ·OH scavenging was studied by 2-deoxyribose oxidation method.The results showed that the optimal separated conditions were detection wavelength 280nm,mobile phase(acetonitrile,methanol and water) at a volume ratio of 5∶5∶90,flow rate 1.0 mL /min,and sampling 20 μL each time.ADGP had 60.19% antioxidant activity at 5.78 μg/mL on·OH scavenging. |
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| Keywords: | Andrias davidianus glycopeptide HPLC OH-scavenging activity |
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