Puroindoline b位点近等基因系对小麦籽粒淀粉含量和组分及其特性的影响 |
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引用本文: | 秦海霞,侯俊峰,丁会纳,夏先春,何中虎,Morris F Craig,王晨阳,马冬云. Puroindoline b位点近等基因系对小麦籽粒淀粉含量和组分及其特性的影响[J]. 华北农学报, 2016, 0(6): 39-43. DOI: 10.7668/hbnxb.2016.06.007 |
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作者姓名: | 秦海霞 侯俊峰 丁会纳 夏先春 何中虎 Morris F Craig 王晨阳 马冬云 |
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作者单位: | 1. 河南农业大学农学院,国家小麦工程技术研究中心,河南郑州450002;2. 中国农业科学院作物科学研究所,国家小麦改良中心,北京100081;3. 美国农业部农业研究中心,西部小麦品质实验室,普尔曼99164-6494 |
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基金项目: | 国家自然科学基金项目(31571651),省部共建小麦玉米作物学国家重点实验室自主课题(39990035) |
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摘 要: | 为了研究不同硬度等位基因与小麦籽粒淀粉组分含量及特性的关系,为小麦品质改良提供依据,以7个硬度Puroindoline b位点近等基因系为试材,研究了不同硬度基因型对小麦籽粒淀粉组分含量及特性的影响。结果表明,Pina-D1b/Pinb-D1a基因型的籽粒硬度值、支链淀粉含量最高;Pina-D1b/Pinb-D1a基因型的淀粉溶解度最高,而Pina-D1a/Pinb-D1d溶解度最低,两者差异达显著水平;不同基因型之间籽粒淀粉酶解力没有显著差异;冻融失水率以基因型Pina-D1a/Pinb-D1b最低,表明Pina-D1a/Pinb-D1b基因型冻融稳定性最好,可能较适宜冷冻食品的制作;对于抗性淀粉,Pina-D1a/Pinb-D1b基因型的抗性淀粉含量最高、Pina-D1a/Pinb-D1d最低,表明Pina-D1a/Pinb-D1b基因型有助于籽粒中抗性淀粉含量的提高。
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关 键 词: | 小麦 Puroindoline基因 近等基因系 淀粉特性 淀粉含量 |
Starch Content and Properties of Common Wheat in Near-isogenic Lines of Puroindoline b Alleles |
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Abstract: | Understanding the effect of Puroindoline b (Pinb) alleles on wheat grain starch content and properties will lay the foundation for improving the wheat quality.Seven wheat near-isogenic lines (NILs) differing in Puroindoline b were used to compare their grain starch component content and properties.The results showed that Pina-D1b/Pinb-D1a genotype possessed significantly higher values of grain hardness and amylopectin content than other genotypes.The Pina-D1b/Pinb-D1a genotype possessed the highest starch solubility,and the Pina-D1a/PinbD1d genotype had the lowest value,their difference being significant.There was no significant difference in starch enzymolysis force between different genotypes.The Pina-D1a/Pinb-D1b genotype had the lowest freezing and thawing loss rate,indicating that Pina-D1a/Pinb-D1b genotype possessed better freeze-thaw stability and would suit for making frozen food.For resistant starch,the Pina-D1a/Pinb-D1b genotype possessed the highest resistant starch content,while the lowest value went to Pina-D1a/Pinb-D1d,which suggested that Pina-D1a/Pinb-D1b genotype contributes to high resistant starch content of grains. |
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Keywords: | Wheat Puroindoline gene Near-isogenic lines Starch properties Starch content |
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