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黑龙江省稻瘟病生理小种及品种资源抗性鉴定
引用本文:辛威,王敬国,孙健,刘化龙,郭丽颖,姜思达,许天宇,赵宏伟,邹德堂. 黑龙江省稻瘟病生理小种及品种资源抗性鉴定[J]. 华北农学报, 2016, 0(4): 130-137. DOI: 10.7668/hbnxb.2016.04.022
作者姓名:辛威  王敬国  孙健  刘化龙  郭丽颖  姜思达  许天宇  赵宏伟  邹德堂
作者单位:东北农业大学 农学院,黑龙江 哈尔滨,150030
基金项目:黑龙江省重大科技招标项目(GA14B102-02)
摘    要:为了明确黑龙江省水稻品种资源稻瘟病抗性,挖掘优异种质资源,适时了解黑龙江省生理小种群体变化特征。采用中国生理小种命名方法,通过苗期喷雾接菌鉴定,将2013-2014年黑龙江省的稻瘟病菌株划分为7个群42个生理小种,优势小种为ZD5和ZD7,出现频率分别为19.77%和12.21%,总频率为31.98%;通过苗期抗病性表现,筛选出宽抗谱品种14份,这些品种携带2~7个抗稻瘟病基因,绥粳12+合江23(Pi9、Pi20、Pi33、Pi54、Pik)、牡丹江26+龙粳31(Pi9、Pi20、Pi33、Pi54、Pita、Pik)、牡丹江26+合江23(Pi9、Pi20、Pi33、Pi54、Pik)等29个在抗稻瘟病育种生产上将具有较好防病效果的组合,并且能够聚合多个抗性基因,提高抗性水平、拓宽抗谱;其中龙粳31与其他9个品种的配对组合均为最优组合,对稻瘟病具有较高抗性;这14份宽抗谱品种是抗稻瘟病育种较好的抗源材料;部分品种如垦稻15、龙粳23和牡丹江25,仅携带2个本研究鉴定的基因,这些品种可能是携带未知抗性基因的新抗源,可作为进一步鉴定和寻找抗性基因的试验材料。

关 键 词:稻瘟病  生理小种  种质资源  稻瘟病抗性  抗性基因

Physiological Races of Rice Blast in Heilongjiang Province and Species Identification of Resistance to Resource
Abstract:Blast resistance of rice varieties in Heilongjiang Province in order to clear resource,mining germ-plasm resources,timely understanding of groups of physiological variation of Heilongjiang Province.Useing the Chi-nese name of physiological races method,by spraying bacteria identification at the seedling stage.The races of rice blast in Heilongjiang Province from 2013 to 2014 can be divided into 7 groups of 42 physiological races,in which the dominant races were ZD5 and ZD7 ,and frequency of occurrence was 19.77% and 12.21 %,respectively.The to-tal frequency was 31 .98%.The result of resistance test in seedling showed that 14 accessions had broad resistance spectrum,these accessions with 2 -7 rice blast resistance genes has been identified,The 29 combinations,such as suijing 12 and Hejiang 23 (Pi9,Pi20,Pi33,Pi54,Pik),Mudanjiang 26 and Longjing 31 (Pi9,Pi20,Pi33,Pi54, Pita,Pik)and Mudanjiang 26 and Hejiang 23 (Pi9,Pi20,Pi33,Pi54,Pik)and so on had better effect of blast re-sistance on breeding production.These comparisons have the quality of,enhancing the level of resistance and broad-ening the spectrum of resistance.The combinations interfered with Longjing 31 and other 9 materials had better re-sistance to rice blast.14 accessions were base materials for multiple resistance genes aggregated in breeding.There may be other unknown genes or new genes in some of the broad spectrum resistant materials,such as Kendao 15, Longjing 23 and Mudanjiang 25 which are containing 2 genes identified in this study,can be used as the test materi-als in further identification and finding resistance genes.
Keywords:Rice blast  Physiological race  Germplasm resources  Blast resistance  Resistance gene
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