转大豆GmPRP和SbPRP基因烟草对非生物胁迫耐受能力分析

为实现PRPs基因在植物抗逆基因工程中的应用,将大豆中的2个脯氨酸富集蛋白基因GmPRP和SbPRP构建到植物表达载体pRI101上,通过叶盘法转化烟草。共获得GmPRP阳性转基因烟草2株,SbPRP阳性转基因烟草4株。对转基因烟草植株进行高盐、干旱和低温胁迫处理。结果表明,高盐处理后,SbPRP转基因烟草的脯氨酸含量和可溶性糖含量分别显著和极显著高于野生型,丙二醛含量极显著低于野生型。干旱处理对GmPRP和SbPRP转基因烟草脯氨酸含量、可溶性糖含量和丙二醛含量的影响均不明显。低温处理后,GmPRP和SbPRP转基因烟草的脯氨酸含量均极显著高于野生型,丙二醛含量均低于野生型。由此推测SbPRP可以提高转基因烟草的耐盐性和耐寒性,GmPRP可以提高转基因烟草的耐寒性,为后续SbPRP和GmPRP的应用奠定基础。

Analysis of Abiotic Stress Resistance Ability of Transgenic Tobacco Expressing GmPRP and SbPRP from Soybean

In order to realize the application of PRPs genes in plant genetic engineering,the GmPRP and Sb-PRP from soybean were cloned into plant expression vector of pRI1 01 ,respectively,and then introduced into tobac-co by Agrobacterium-mediated transformation.Two positive transgenic tobacco plants of GmPRP and four positive transgenic tobacco plants of SbPRP were obtained.The transgenic tobacco plants were treated with salt,drought and cold stresses.The results showed that the contents of proline and soluble sugar in SbPRP transgenic plants were sig-nificant higher than wild-type plants and the contents of malondialdehyde in SbPRP transgenic plants were signifi-cant lower than wild-type plants under salt stress.While the contents of proline,soluble sugar,and malondialdehyde in GmPRP and SbPRP transgenic plants had no significant differences compared to wild-type plants under drought stress.The contents of proline in GmPRP and SbPRP transgenic plants were both significant higher than wild-type plants and the contents of malondialdehyde in GmPRP and SbPRP transgenic plants were both lower than wild-type plants under cold stress.These data indicated that the transgenic tobacco plants constitutively expressing SbPRP showed an increased tolerance to salt and cold stresses and the transgenic tobacco plants constitutively expressing GmPRP showed an increased tolerance to cold stress,which may provide a way toward the application of them.