转水稻OsSIK1基因玉米植株的获得及抗旱性分析

类受体激酶基因Os SIK1具有通过激活抗氧化系统,增强水稻对于干旱和盐胁迫抗性的作用。为了丰富可利用的作物抗旱基因,获得具有较高抗旱水平的玉米新种质,通过超声波辅助花粉介导法,将水稻类受体激酶基因Os SIK1导入玉米自交系郑58中,并对转化株进行卡那霉素筛选及T1、T2、T3的PCR及Southern Blotting杂交等分子检测,获得转化植株并在T3获得转基因纯合株系。对T3转基因玉米和非转基因玉米对照以16.1%的PEG模拟水分胁迫进行抗旱性分析。结果表明,与对照相比,在水分胁迫处理下,转基因玉米株系叶片相对含水量提高了7.4%~19.8%,叶绿素含量提高了11.3%~106.9%,SOD活性上升45.8%~93.4%,而转基因玉米叶片的相对电导率下降了35.4%~58.1%,MDA含量下降了25.7%~50.4%,说明转Os SIK1基因玉米植株抗旱性得到提高,其中,5个转化株系与对照在抗旱性方面有显著差异,且生长状况明显优于对照。综上所述,研究最终获得5个转Os SIK1基因玉米株系,并证明导入水稻Os SIK1基因可以提高玉米植株的抗旱性。

Analysis on Obtaintion of Transgenic Maize Plants Transferred with OsSIK1 Gene and Their Drought Resistance

A Receptor-like kinase (RLK)gene,OsSIK1,plays important roles in drought stress-tolerance in rice,through the activation of the antioxidative system.To make maize plants have much more drought resistance genes and further to obtain drought tolerance maize germplasm.In this study,OsSIK1 gene of rice was transformated into maize inbred Zheng 58 plants by pollen-mediated method.First,transgenic plants in T1 ,T2 and T3 were detected by Kanamycin resistance screening,PCR and Southern Blotting,transgenic plants were obtained from T1 and pure transgenic lines was obtained from T3 .Next,drought resistance analysis to transgenic maize plants and non-transfor-mation control plants were conducted under the condition of 1 6.1 % PEG drought stress.The results showed that compared with non-transgenic plants,the seedling leaf relative water content,chlorophyll content and SOD activity of transgenic plants were increased by 7.4%-1 9.8%,1 1 .3%-1 06.9% and 45.8%-93.4%,respectively;further-more,the relative conductivity and MDA content were decreased by 35.4%-58.1 %,and 25.7%-50.4%,respec-tively.All the physiological indexes under the drought stress proved that transgenic OsSIK1 gene improved the drought resistance of transgenic maize plants,further analysis found that there were significant differences on drought tolerance between 5 transformed lines and their control groups,and their performance of field were superior to that of non-transgenic maize seedlings.At last,5 transgenic maize inbred lines were obtained,which suggested that geneti-cally modified maize has improved the drought resistance by introducing foreign OsSIK1 gene of rice.