Translation of double-stranded RNA segments 1 and 2 of Chuzan virus in rabbit reticulocyte lysates. |
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| Authors: | T Yoshida R Harasawa Y Goto |
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| Institution: | Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan. |
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| Abstract: | Coding assignments of genome segments 1 and 2 of Chuzan virus strain K-47 were studied in vitro by using rabbit reticulocyte lysates. The double-stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)-polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with 35S]methionine were resolved by SDS-polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively. |
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