| 舌状蜈蚣藻多糖提取工艺及抗氧化活性分析 |
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| 引用本文： | 陈胜军,刘欢,杨少玲,杨贤庆,戚勃,胡晓. 舌状蜈蚣藻多糖提取工艺及抗氧化活性分析[J]. 上海海洋大学学报, 2020, 29(1): 153-160 |
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| 作者姓名： | 陈胜军 刘欢 杨少玲 杨贤庆 戚勃 胡晓 |
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| 作者单位： | 中国水产科学研究院南海水产研究所 国家水产品加工技术研发中心,农业农村部水产品加工重点实验室,广东 广州 510300;江苏省海洋生物产业技术协同创新中心,江苏 连云港 222005;中国水产科学研究院南海水产研究所 国家水产品加工技术研发中心,农业农村部水产品加工重点实验室,广东 广州 510300;上海海洋大学 食品学院,上海201306;中国水产科学研究院南海水产研究所 国家水产品加工技术研发中心,农业农村部水产品加工重点实验室,广东 广州 510300 |
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| 基金项目： | 中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金（2017ZD03）；现代农业产业技术体系专项资金（CARS－50）；农业部财政重大专项（NFZX2013）；广东省海洋与渔业发展专项资金（2017A0001） |
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| 摘 要： | 在单因素实验的基础上,以舌状蜈蚣藻多糖得率为指标,选择料液比、温度和时间进行响应面实验,确定最佳工艺条件,同时测定舌状蜈蚣藻多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基以及羟自由基(·OH)的清除能力。结果显示,舌状蜈蚣藻多糖的最佳提取工艺为料液比1∶37 g/mL,提取温度100℃,提取时间4 h,此时的多糖得率为15.23%。舌状蜈蚣藻多糖清除DPPH自由基和羟自由基的半抑制质量浓度(IC_(50))分别为12.61 mg/mL和2.05 mg/mL,具有较好的体外抗氧化活性。
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| 关 键 词： | 舌状蜈蚣藻 多糖 响应面 抗氧化活性 |
| 收稿时间： | 2018-10-18 |
| 修稿时间： | 2019-06-28 |
Extraction and antioxidant activity of polysaccharides from Grateloupia livida |
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| CHEN Shengjun,LIU Huan,YANG Shaoling,YANG Xianqing,QI Bo and HU Xiao. Extraction and antioxidant activity of polysaccharides from Grateloupia livida[J]. Journal of Shanghai Ocean University, 2020, 29(1): 153-160 |
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| Authors: | CHEN Shengjun LIU Huan YANG Shaoling YANG Xianqing QI Bo HU Xiao |
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| Affiliation: | National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China,National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;College of Food Science&Technology, Shanghai Ocean University, Shanghai 201306, China,National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China,National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China and National Research and Development Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China |
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| Abstract: | |
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| Keywords: | Grateloupia livida polysaccharides response surface methodology antioxidant activity |
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