木醋液对滨海盐土解磷真菌Apophysomyces sp.SM-1生长代谢及其溶磷效果的影响

为缓解不溶性磷酸盐累积造成的盐碱土壤板结问题,从江苏滨海盐碱土壤中筛选到一株具有较强溶磷能力的真菌菌株SM-1,并初步鉴定为Apophysomyces sp.,同时考察了木醋液对该菌生长及溶磷效果的影响。结果表明,木醋液的添加能够显著影响菌株SM-1的生长,添加量为0.1%的木醋液是其最适宜的生长浓度。菌株SM-1对不同磷源有着不同的分解能力,其中对磷酸三钙的分解能力最佳,培养基中水溶磷含量由93 mg·L~(-1)提高到246.9 mg·L~(-1)。木醋液对供试磷源没有分解效果,但添加木醋液则能够显著提高菌株对磷矿粉的分解能力,培养基中水溶磷含量由79 mg·L~(-1)提高到115.3 mg·L~(-1),而对磷酸三钙的分解能力与单独接入菌株SM-1的效果相当。这预示着菌株SM-1与木醋液混合使用在改善盐碱化土壤肥力方面具有很好的应用前景。

Effect of pyroligneous acid on growth,metabolism and phosphate-dissolving ability of phosphate-solubilizing fungi Apophysomyces sp. SM-1

In order to alleviate the problem of the hardened soil due to the accumulation of insoluble phosphate, a phosphate-solubilizing fungi strain SM-1 had been isolated from the Jiangsu seashore saline soil. Strain SM-1 was identified as Apophyso-myces sp. , and how its growth, metabolism and phosphate-dissolving ability were affected by pyroligneous acid was tested. The results showed that strain SM-1 grew the best under 0. 1% pyroligneous acid concentration. The strain SM-1 could decom-pose different phosphorus forms, especially for the tricalcium phosphate. The available phosphate was improved from 93 mg?L-1 to 246. 9 mg?L-1 . Although pyroligeneous acid didn't dissolve the tested phosphate resource, but it promoted the solubilizing capability of strain SM-1 in the liquid phosphate rock powder. Strain SM-1 improved the available phosphate from 79 mg?L-1 to 115. 3 mg?L-1 . In conclusion, phosphate-solubilizing fungi strain SM-1 mixed with pyroligeneous acid had great potential to improve phosphorus utilization rate in saline soil.