| 小麦材料PI31抗条锈性鉴定及其抗性基因SSR标记 |
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| 引用本文： | 蒲宗君,颜泽洪,魏育明,杨武云,郑有良,张增艳. 小麦材料PI31抗条锈性鉴定及其抗性基因SSR标记[J]. 植物病理学报, 2006, 36(4): 342-346 |
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| 作者姓名： | 蒲宗君 颜泽洪 魏育明 杨武云 郑有良 张增艳 |
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| 作者单位： | 1 四川农业大学小麦研究所, 都江堰 611830;2 四川省农业科学院作物研究所, 成都 610066;3 中国农业科学院作物科学研究所, 北京 100081 |
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| 基金项目： | 四川省科技攻关项目;国家高技术研究发展计划(863计划);四川省应用基础研究计划;四川省科技厅资助项目 |
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| 摘 要： | 小麦材料PI31对我国当前流行的条锈菌小种条中30、31和32免疫;遗传分析表明,PI31携带一个显性抗条锈病基因。等位性测定显示,PI31所携带的抗条锈病基因与已知抗锈基因Yr5、Yr10和Yr15不等位。抗源系谱分析表明,该基因来源于叙利亚普通小麦品系叙18;故将此材料携带的抗条锈病基因暂定名为Yr-XU。利用分组分析(BSA)法,筛选到1个位于1 BS的SSR标记WM S11-193 bp片段与Yr-XU紧密连锁,将Yr-XU定位于小麦1BS上;对F2分离群体142个单株分析结果表明,Yr-XU与WM S11-193 bp的遗传距离为2.1 cM,可将此标记用于小麦抗条锈病分子标记辅助育种。
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| 关 键 词： | 小麦条锈病 鉴定 等位测试 SSR标记 辅助选择 |
| 文章编号： | 0412-0914(2006)04-0342-05 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005-06-13 |
Identification and SSR mapping of a stripe rust resistance gene in wheat line PI31 |
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| PU Zong-jun,YAN Ze-hong,WEI Yu-ming,YANG Wu-yun,ZHENG You-liang,ZHANG Zeng-yan. Identification and SSR mapping of a stripe rust resistance gene in wheat line PI31[J]. Acta Phytopathologica Sinica, 2006, 36(4): 342-346 |
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| Authors: | PU Zong-jun YAN Ze-hong WEI Yu-ming YANG Wu-yun ZHENG You-liang ZHANG Zeng-yan |
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| Affiliation: | 1 Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China;2 Crop Research Institute, Sichuan Academy of Agriculture Science, Chengdu 610066, China;3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | A wheat line,PI31,carried a novel single dominant candidate gene conferring high resistance to stripe rust isolates CYR30,CYR31 and CYR32.By using allelic test and pedigree analysis,it was found that the resistance gene in PI31 derived from Syria common wheat line Xu18 and was different from Yr5,Yr10 and Yr15.This novel candidate gene was designated as Yr-XU.A SSR maker,WMS11-193 bp,on the 1BS was associated with Yr-XU.The genetic distance between Yr-XU and WMS11-193 bp was about 2.1 cM.Therefore,WMS11 could be used as molecular marker assisted selection for Yr-XU and for pyramiding with other stripe rust resistance genes in wheat breeding program. |
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| Keywords: | stripe rust identification allelic test SSR marker assisted selection |
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