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| 苦参和狼牙刺植株中苦参碱与氧化苦参碱的含量测定 | |
| 引用本文： | 江海,吴三桥,江珊,田姗,韩豪.苦参和狼牙刺植株中苦参碱与氧化苦参碱的含量测定[J].湖北农业科学,2009,48(12). |
| 作者姓名： | 江海 吴三桥 江珊 田姗 韩豪 |
| 作者单位： | 1. 陕西理工学院,陕西省资源生物重点实验室陕西,汉中,723001;陕西理工学院,生物科学与工程学院,陕西,汉中,723001 2. 陕西理工学院,生物科学与工程学院,陕西,汉中,723001 3. 四川省高县中学,四川,高县,645150 4. 陕西省汉中市3201医院,陕西,汉中,723000 5. 陕西理工学院,陕西省资源生物重点实验室陕西,汉中,723001 |
| 基金项目： | 陕西省教育厅资助项目，陕西理工学院资助项目 |
| 摘 要： | 采用高效液相色谱法对野生苦参和狼牙刺各组织器官中苦参碱和氧化苦参碱含量进行测定.色谱.柱ZORBAX SB-C18(150mm×4.6mm,5μm),检测波长:220 am,流动相:0.04mol/L磷酸-甲醇(90:10),流速:1.0 mL/min,柱温:20℃.结果表明,野生苦参的苦参碱舍量较高,作为药用部分的苦参根,其苦参碱含量达到0.34%;狼牙刺的氧化苦参碱含量较高,其种子中氧化苦参碱含量达到2.45%.狼牙刺植株在苦参类生物碱开发利用方面有较高利用价值. |
| 关 键 词： | 苦参 狼牙刺 苦参碱 氧化苦参碱 |
Determination of Matrine and Oxymatrine in Sophora flavescens Alt and Sophora viciifol |
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| JIANG Hai,WU San-qiao,JIANG Shan,TIAN Shan,HAN Hao.Determination of Matrine and Oxymatrine in Sophora flavescens Alt and Sophora viciifol[J].Hubei Agricultural Sciences,2009,48(12). | |
| Authors: | JIANG Hai WU San-qiao JIANG Shan TIAN Shan HAN Hao |
| Abstract: | The contents of matrine and oxymatrine in the wildness Sophora flavescens Alt and Sophora viciifoli were detected by HPLC with ZORBAX SB-C18 (150mm×4.6mm,5μm) column at 20℃.The mobile phase coasisted of 0.04 mol/L H_3PO_4 (pH=1.8)-CH_3OH (90:10).The flow rate Was 1.0 mL/min and detection wave lensth Was at 220 nm.The resuIts revealed that the high content of matrine in the wild Sophora flavescens Alt especially in its root was observed.The cantent of matrine in root reached 0.34%.On the other hand,2.45% oxymatrine in the Sophora viciifoli seed which was higher than Sophora flavescens Alt indicated that Sophora viciifoli had a good utilization value for producing matrine. |
| Keywords: | sophora flavescens Alt sophora viciifoli matrine oxymatrine |
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