Amylolytic activity in stored potato tubers. 1. Estimation usingp-nitrophenyloligosaccharides |
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| Authors: | M Patricia Cochrane Carol M Duffus M J Allison G R Mackay |
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| Institution: | (1) Edinburgh School of Agriculture, West Mains Road, EH9 3JG Edinburgh, Scotland, UK;(2) Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland UK;(3) Present address: Department of Biological Sciences, Heriot-Watt University, Ricearton, EH14 4AS Edinburgh, Scotland, UK |
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| Abstract: | Summary Methods for the estimation of amylolytic activity are reviewed. A procedure for the routine extraction of amylolytic activity
from freeze-dried powder prepared from potato tubers is described. The extraction medium is buffered at pH 7.0 and contains
glycerol, dithiothreitol, calcium chloride and the non-ionic detergent, nonidet P-40. α-Amylase activity and exoamylolytic
activity were estimated in crude extracts of potato tubers using the substrates, blockedp-nitrophenyl-maltoheptaoside andp-nitrophenyl-maltopentaoside respectively. These substrates are included in kits supplied by Biocon, (UK) Ltd to measure the
α- and ?-amylase activity in cereals. The validity of using these kits for the determination of α- and ?-amylase activity
in potato tuber tissue is discussed. |
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| Keywords: | α -amylase ?-amylase α -glucosidase Solanum tuberosum L |
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