| CSFV和PRRSV双重RT-PCR检测方法的建立及其应用 |
| |
| 引用本文： | 谢金文,沈志强,管宇,韩玲,任艳玲,曲光刚. CSFV和PRRSV双重RT-PCR检测方法的建立及其应用[J]. 安徽农业大学学报, 2009, 36(2) |
| |
| 作者姓名： | 谢金文 沈志强 管宇 韩玲 任艳玲 曲光刚 |
| |
| 作者单位： | 山东省滨州畜牧兽医研究院,滨州,256600;山东省滨州畜牧兽医研究院,滨州,256600;山东绿都生物科技有限公司,滨州,256600 |
| |
| 摘 要： | 为建立一种能够同时快速诊断猪瘟(CSF)和猪繁殖与呼吸综合征(PRRS)2种传染病的方法,根据GenBank上已发表的猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)核苷酸序列,分别设计并合成2对能特异性扩增CSFV、PRRSV的引物,经过条件优化后,建立了检测CSFV、PRRSV的双重RT-PCR方法,扩增2种病毒的片段,大小分别为508bp和432bp.试验证明,该方法具有良好的特异性、敏感性、重复性和稳定性,简便、快速、高效,为CSFV与PRRSV的快速诊断及进一步的CSFV与PRRSV的流行病学研究提供了重要的技术支持.
|
| 关 键 词： | 猪瘟病毒 猪繁殖与呼吸综合征病毒 双重RT-PCR |
Establishment and application of a double RT-PCR for classical swine fever virus and porcine reproductive and respiratory syndrome virus |
| |
| XIE Jin-wen,SHEN Zhi-qiang,GUAN Yu,HAN Ling,REN Yan-ling,QU Guang-gang. Establishment and application of a double RT-PCR for classical swine fever virus and porcine reproductive and respiratory syndrome virus[J]. Journal of Anhui Agricultural University, 2009, 36(2) |
| |
| Authors: | XIE Jin-wen SHEN Zhi-qiang GUAN Yu HAN Ling REN Yan-ling QU Guang-gang |
| |
| Affiliation: | 1.Shandong Binzhou Animal Science & Veterinary Medicine Academy;Binzhou 256600;2.Shandong Lvdu Bio-Science & Technology Co.Ltd;Binzhou 256600 |
| |
| Abstract: | According to the nucleotide sequences of classical swine fever virus(CSFV)and porcine reproductive and respiratory syndrome virus(PRRSV)in GenBank,two pairs of primers were designed and synthesized.A double RT-PCR was developed based on these two pairs of primers which amplified the CSFV virus-specific segment with 508bp and the PRRSV virus-specific segment with 432bp in sizes after the conditions of PCR were optimized.The results indicate that the double RT-PCR reaction after optimization show a good sensi... |
| |
| Keywords: | classical swine fever virus porcine reproductive and respiratory syndrome virus double RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
|
点击此处可从《安徽农业大学学报》下载全文 |
|
