越橘葡萄座腔菌枝枯病的病原菌鉴定

 通过形态学观察、诱导产孢、致病力测定和分子生物学研究，对引起辽宁地区越橘枝枯病 的病原菌进行鉴定，确定其为葡萄座腔菌（Botryosphaeria dothidea）。病原菌在越橘植株上接种后表现枝 条枯萎、木质部变色等症状，与田间自然发病植株一致；在PDA 培养基上菌落初期为白色，后转为灰黑 色，分生孢子纺锤形或梭形，无色，单胞，内含多个油滴；水琼脂 + 麦芽提取物、苹果枝条和苹果幼果 3 种病原菌培养方法均可诱导产孢，苹果幼果是诱导病原菌产孢的最佳基质。供试菌株LB1 的rDNA-ITS 和β-tubulin 基因序列与GenBank 中已有的Botryosphaeria dothidea 序列相似性分别达到99%与100%。

Research on the Pathogen Causing Botryosphaeria Stem Blight on Blueberry

The pathogen causing stem blight on blueberry in Liaoning，China was identified as Botryosphaeria dothidea by morphological characteristics，sporulation features，pathogenicity test，and the molecular identification. The stems showed symptoms of dieback，discoloration in trunks and death after inoculating with the pathogen，which were consistent with the symptoms of the natural infected plants. All isolates showed the colonies white at the early stage，then became gray to black，conidia were hyaline， aseptate，fusiform with several oil globules，young apple fruit is the suitable medium for sporulation. Identity of isolate LB1 was confirmed by analysis of the rDNA-ITS and β-tubulin sequences with primers ITS1–ITS4 and Bt2a–Bt2b. BLAST searches showed 99% and 100% identity with Botryosphaeria dothidea isolates from GenBank.