| 非洲紫罗兰愈伤组织诱导及植株再生 |
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| 引用本文： | 龚宇舟,刘清波,雷洁琼,夏明,廖兵辉.非洲紫罗兰愈伤组织诱导及植株再生[J].农业工程,2013,3(3):120-122. |
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| 作者姓名： | 龚宇舟 刘清波 雷洁琼 夏明 廖兵辉 |
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| 作者单位： | 1.湖南农业大学生物科学技术学院，长沙410128 |
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| 基金项目： | 湖南农业大学大学生研究性学习与创新性实验计划(项目编号:XCX12026) |
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| 摘 要： | 以非洲紫罗兰为试验材料，以其无菌苗叶片为外植体，建立了愈伤组织诱导及植株再生体系。试验结果表明:MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基配方诱导愈伤组织效果最好，出愈率可达73.9%，暗培养不利于愈伤组织诱导；在培养基MS+6-BA 1.0mg/L+NAA 0.1mg/L中愈伤组织分化不定芽的分化系数最高可达14.0；MS+NAA 0.5mg/L+IAA 0.5mg/L培养基可使生根率达到100%，平均单株生根数达12.4。
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| 关 键 词： | 非洲紫罗兰 愈伤组织 植株再生 |
Callus Induction and Plant Regeneration of Saintpaulia ionantha |
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| Gong Yuzhou,Liu Qingbo,Lei Jieqiong,Xia Ming and Liao Binghui.Callus Induction and Plant Regeneration of Saintpaulia ionantha[J].Agricultural Engineering,2013,3(3):120-122. |
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| Authors: | Gong Yuzhou Liu Qingbo Lei Jieqiong Xia Ming and Liao Binghui |
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| Abstract: | Callus induction and plant regeneration system were constructed by using leaves of sterile Saintpaulia ionantha as explants.Results showed callus induction ratio were 73.9% and induction efficiency was best in the medium MS+6-BA 2.0mg/L+NAA 0.5mg/L.Darkness is not conducive to callus induction.Differentiation coefficient of callus was highest up to 14.0 in the medium MS+6-BA 1.0mg/L+NAA 0.1mg/L.Rooting percentage was up to 100% in MS+NAA 0.5mg/L+IAA 0.5mg/L medium，the average roots of each plant were 12.4. |
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