| 热带土壤解磷细菌PSB26的筛选鉴定及拮抗初探 |
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| 引用本文： | 王义,余贤美,郑服丛.热带土壤解磷细菌PSB26的筛选鉴定及拮抗初探[J].安徽农学通报,2009,15(7):59-62. |
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| 作者姓名： | 王义 余贤美 郑服丛 |
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| 作者单位： | 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,海南儋州,571737;农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州,571737海南省热带农业有害生物检测监控重点实验室,海南儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资助项目，国家科技支撑计划，公益性行业(农业)科研专项 |
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| 摘 要： | 以有机磷液体培养基进行生物富集,无机磷固体培养基平板法从海南、广东、云南等地热带土壤中筛选出解磷效果较好的一株解磷细菌PSB26。通过细菌16S rRNA通用引物PCR扩增菌株的16SrDNA序列,测得该菌株的序列长度为1095bp,经B lastn搜索进行序列比对,结果表明：该菌株属于Klebsiella sp.。进一步的室内平板对峙培养实验表明,PSB26对Fusarium oxysporum piperis等11个供试病原菌中的6个具有不同程度的拮抗作用。
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| 关 键 词： | 热带土壤 解磷细菌 16SrDNA 分子鉴定 拮抗 |
Screening of Phosphate Solubilizing Bacterias(PSB26) from the Tropical Soils and the Study of its Antagonism |
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| Institution: | Wang Yi et al. ( 1 Environment and Plant Protection College, Hainan University, Danzhou 571737,China ;2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Baleful Organism Detection and Monitor Centre for Tropical Agriculture, Danzhou 571737, China;3 Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests , Ministry of Agriculture, Danzhou 571737, China;4 Key Laboratory of Pests Detection and Control for Tropical Agriculture of Hainan Province, Danzhou 571737 ,China) |
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| Abstract: | The screening system of phosphorus solubilizing bacteria in the tropical soil was established by biological enriching using organic phosphobacteria liquid medium and then screening via plate dilution method using inorganic phosphobacteria solid medium.By 16S rDNA sequencing technique,the strain PSB26 with the 16SrDNA gene of 1095 bp length was identified as Klebsiella sp.by Blastn search in NCBI database.The results of in-door antagonism experiment of PSB26 to 11 strains of Fusarium oxysporum piperis et sho... |
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| Keywords: | 16SrDNA |
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