| 利用酚试剂测定漆酶活性的比色法 |
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| 引用本文： | 周易勇,郭明高. 利用酚试剂测定漆酶活性的比色法[J]. 林产化学与工业, 1987, 0(4) |
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| 作者姓名： | 周易勇 郭明高 |
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| 作者单位： | 湖北省化学研究所 武汉(周易勇)，湖北省化学研究所 武汉(郭明高) |
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| 摘 要： | 利用酚试剂研究了漆酶活性的比色测定方法。在实验条件下焦性没食子酸氧化产物的吸光值倒数与反应时间的倒数成正比,符合公式1/Q=(b/a)(1/t)+1/a。于是可用上式中a/b来表征漆酶的催化活性。实测数据表明,本法比常规比色法和通气比色法灵敏。
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| 关 键 词： | 漆酶 焦性没食子酸 中国生漆 酚试剂 丙酮粉末 |
COLORIMETRIC METHOD FOR DETERMINING LACCASE ACTIVITY USING 3-METHYL-2- BENZOTHIAZOLINONE HYDRAZONE HYDROCHLORIDE |
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| Zhou Yiyong Guo Minggao. COLORIMETRIC METHOD FOR DETERMINING LACCASE ACTIVITY USING 3-METHYL-2- BENZOTHIAZOLINONE HYDRAZONE HYDROCHLORIDE[J]. Chemistry & Industry of Forest Products, 1987, 0(4) |
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| Authors: | Zhou Yiyong Guo Minggao |
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| Affiliation: | Hubei Research Institute of Chemistry Wuhang |
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| Abstract: | Colorimetric method for determining laccase activity using 3-methyl-2-benzothiazolinone hydrazone hydrochloride was studied. Under experimental conditions, the reciprocal of photoabsorbtion value(Q) is direct proportional to reciprocal of reaction time(t), which is in agreement with the following formula 1/Q =b/a( 1/t) + 1/a. Hence, the factor a/b in it can be used to characterize the catalytic activity of the laccase. It is obvious from the data obtained in this work that this method is more sensitive than both normal colorimetry and aerate colorimetry for determining laccase activity. |
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| Keywords: | Laccase Pyrogallic acid Chinese raw lacquer 3-methyl- 2-benzothiazolinone hydrazone hydrochloride Lacquer po- wder in acetone |
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