香蕉束顶病毒海南分离物DNA组分的克隆及序列分析

摘要：以中国海南岛地区表现束顶病毒(Banana bunchy top virus, BBTV)症状的香蕉组织总DNA为模板， 通过PCR方法克隆了BBTV海南分离物的6个DNA组分全序列，并进行了核苷酸序列分析。测序结果表明， DNA1~6序列全长分别为1104、1067、1059、1045、1014和1081 nt，均已登录到GenBank(登录号分别为AY450396、AY606084、AY494786、AY494788、AY606085和AY494787)。与世界不同地域分离物进行序列同源分析，发现DNA1序列较保守，与越南分离物的同源性达到了95.4%；而DNA2和DNA4变异较大，其中DNA2序列与广州分离物的同源性仅有83%。 根据DNA1的序列差异分析初步确定BBTV海南分离物属于亚洲亚组。

Cloning and Sequencing of DNA Components of Banana bunchy top virus Hainan Isolate

Abstract：Six DNA components of Banana bunchy top virus （BBTV）were amplified by PCR using total DNA from banana tissues which are of characteristic of typical BBTV symptom as template. The sequence analysis indicated that the 6 fragments were all full-length components of BBTV, they were 1 104, 1 067, 1 059, 1 045, 1 014 and 1 081 nt respectively. All of them have been submitted to GenBank(Accession No. AY450396, AY606084, AY494786, AY494788, AY606085 and AY494787, respectively). The viral sequences from different countries were compared. Results revealed that the sequence of component 1 was more conservative than other components, DNA1 of Hainan isolate shared homologies of 95.4% with that of Vietnam isolate, while both the sequences of component 2 and component 4 had more difference, and DNA2 of Hainan isolate only shared homologies of 83% with that of Guangzhou isolate. According to DNA1 sequence comparison results, the BBTV Hainan isolate should belong to Asia sub-group.