红腺忍冬腋芽萌发与诱导研究（英文）

以广西山银花的种植品种红腺忍冬带腋芽茎段为外植体,研究不同灭菌方法、取材季节、激素和椰子汁对腋芽萌发或增殖的影响。结果表明,较适宜的外植体灭菌处理为75%酒精浸泡25 s,再用0.1%升汞溶液（加吐温-80）处理6 min,腋芽萌发率可达到53.3%;在春季取的外植体,其灭菌效果最好,腋芽培养7 d即可萌发,而以秋季取的外植体灭菌效果最差,腋芽需培养12 d才可萌发。腋芽萌发诱导较适宜的培养基为MS＋6-BA 3.0 mg/L＋KT 0.5 mg/L,腋芽增殖继代培养较适宜的培养基为MS＋6-BA 2.0 mg/L＋KT 0.5 mg/L。

Germination and induction of axillary buds in micropropagated Lonicerae hypoglauca

The shoot of Lonicerae hypoglauca cuhivar (Lonicerae confusa DC) with axillary buds was used as explant to study the effects of different stefihzation methods and sampling seasons, and different concentrations of hormones and active ingredient on the germination, induction and proliferation of axillary buds in micropropagated shoots. The sterilization treatment with 75% alcohol for 25 s combined with dipping shoots in 0.1% HgCl2 solution with tween-80 for 6 rain wasfound to be the most effective, as shown by 53.3% germination rate of axillary buds. Further, the sterilization effects were found to be most effective in spring sampled explants, which germinated after 7 days of culture, compared to 12 days in autumn samples. The suitable culture medium for germination and proliferation of axillary buds were found as follows: MS media+6-BA (3.0 mg/L) + KT(0.5 rag/L), and MS media+6-BA (2.0 mg/L) + KT(0.5 mg/L), respectively.