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广西油茶炭疽病病原菌鉴定及生物学特性
引用本文:朱英芝,廖旺姣,邹东霞,吴耀军,邓艳.广西油茶炭疽病病原菌鉴定及生物学特性[J].植物保护学报,2015,42(3):382-389.
作者姓名:朱英芝  廖旺姣  邹东霞  吴耀军  邓艳
作者单位:广西壮族自治区林业科学研究院,国家林业局中南速生材繁育实验室,广西优良用材林资源培育重点实验室,南宁530002
基金项目:广西林业科技项目(桂林科字[2012]9号),广西林科院基本科研业务费(林科201420号)
摘    要:为明确广西油茶炭疽病病原菌种类及其生物学特性,通过形态学方法对分离获得的病原菌进行初步鉴定,以核糖体内转录间隔区、微管蛋白基因、几丁质合成酶基因、肌动蛋白基因、3-磷酸甘油醛脱氢酶基因进行多基因系统分析鉴定;并用十字交叉法、菌丝干重法和血球计数法对其生物学特性进行研究。结果表明,经形态学和多基因联合系统结合分析鉴定,广西油茶炭疽病病原菌为胶孢炭疽菌Colletotrichum gloeosporioides Penz.。该病原菌生长温度范围为10~35℃,最适生长温度为28℃;产孢温度范围为15~32℃,最适产孢温度为32℃;生长p H范围为3~11,最适生长p H为4;可促进菌丝生长并适合产孢的碳源为菊糖,阿拉伯树胶粉抑制菌丝生长但可促进产孢;对菌丝生长及产孢均有促进作用的氮源为酵母粉和牛肉膏,而硫酸铵和硝酸铵则表现抑制作用。

关 键 词:油茶  炭疽病  胶孢炭疽菌  生物学特性
收稿时间:2014/4/29 0:00:00

Identification and biological characteristics of the pathogen from Camellia oleifera anthracnose in Guangxi
Zhu Yingzhi,Liao Wangjiao,Zou Dongxi,Wu Yaojun and Deng Yan.Identification and biological characteristics of the pathogen from Camellia oleifera anthracnose in Guangxi[J].Acta Phytophylacica Sinica,2015,42(3):382-389.
Authors:Zhu Yingzhi  Liao Wangjiao  Zou Dongxi  Wu Yaojun and Deng Yan
Institution:Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation; Forestry Research Institute of Guangxi Zhuang Autonomous Region; Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi Zhuang Autonomous Region, China;Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation; Forestry Research Institute of Guangxi Zhuang Autonomous Region; Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi Zhuang Autonomous Region, China;Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation; Forestry Research Institute of Guangxi Zhuang Autonomous Region; Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi Zhuang Autonomous Region, China;Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation; Forestry Research Institute of Guangxi Zhuang Autonomous Region; Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi Zhuang Autonomous Region, China;Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation; Forestry Research Institute of Guangxi Zhuang Autonomous Region; Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi Zhuang Autonomous Region, China
Abstract:
Keywords:Camellia oleifera  anthracnose  Colletotrichum gloeosporioides Penz    biological characteristics
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