丛枝菌根菌丝桥传递作用对烟草抗病性相关酶活性的影响

为明确供体与受体植株间菌丝桥传递抗病信号对受体植株生长以及抗病性相关酶活性的影响,利用丛枝菌根真菌在供体与受体烟苗植株间建立菌根菌丝桥,对供体植株接种青枯菌的方法进行研究。结果表明:在供体烟苗接种丛枝菌根真菌条件下,再接种青枯菌,比只有菌丝连接的受体烟苗叶片内的过氧化物酶(peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、苯丙氨酸解氨酶(phenylalanine ammonialyas,PAL)的活性分别提高了21%、29%和14%,地上部干重和植株磷含量也有相同的趋势;而在不接种丛枝菌根真菌条件下,供体烟苗接种青枯菌与否对酶的活性、植株干重及氮、磷含量均无显著影响。表明供体烟苗产生的抗病信号可以通过菌丝桥传递给受体烟苗,增强受体烟苗的抗病性。

The effect of transfer between roots of different tobacco plants through common arbuscular mycorrhiza networks on enzyme activities related to disease resistance

To determine whether the transformation of common mycorrhizal networks (CMN) between the "donor" and "receiver" plant roots can improve the disease-resistance of donor plants, tobacco (Nicotiana tabacum) plants were inoculated with arbuscular mycorrhizal fungus (AMF) Glomus mosseae to establish CMN between "donor" and "receiver" plants and then inoculated the donor plants with Ralstonia solanacearum, the causal agent of tobacco green wilting disease. The results showed that the activities of phenylalanine ammonialyas, polyphenol oxidase, and peroxidase in the "receiver" plants connected with the donor plants by CMN increased 21%, 29%, and 14%, respectively, when inoculated with R. solanacearum compared with the control, and the same to shoot dry weight and phosphorus concentration. But the effect of inoculation with R.solanacearum on the enzyme was not significant when CMN was not present. These findings indicated that the disease resistance signals produced by the pathogen-induced donor plant could be transferred to the receiver plant roots through CMN.