新疆三种葡萄病毒RT-PCR检测及序列分析

为研究新疆葡萄中沙地葡萄茎痘伴随病毒(Grapevine rupestris stem pitting associated virus,GRSPa V)、葡萄斑点病毒(Grapevine fleck virus,GFk V)及葡萄病毒A(Grapevine virus A,GVA)的发生情况和新疆分离株系统进化关系,分别克隆3种病毒新疆分离株部分基因区域,应用RT-PCR对新疆64份葡萄样品中上述3种病毒进行检测,并进行系统进化分析。结果显示,GRSPa V、GFk V和GVA的检出率分别为31.3%、62.5%和25.0%。新疆GRSPa V分离株(KJ801847)与美国GRSPa V分离株(AY368590)同源性达96.59%;新疆GFk V分离株(KJ801846)与日本GFk V分离株(AB222861)及中国辽宁GFk V分离株(JF927942)的同源性分别为91.70%和91.03%;新疆GVA分离株(KJ801845)与波兰GVA分离株(JN860997)同源性为93.88%,与中国四川GVA分离株(HQ671655)及辽宁GVA分离株(FJ445220)的同源性分别为92.92%和89.53%。表明3种葡萄病毒在新疆发生比较普遍,且新疆分离株与国内其它地方的分离株存在较大差异。

RT-PCR detection and sequence analysis on three grapevine viruses from Xinjiang Uyghur Autonomous Region

To detect the grape virus, including Grapevine rupestris stem pitting associated virus (GRSPaV), Grapevine fleck virus (GFkV), Grapevine virus A (GVA), and evaluate their phylogenetic relationships with isolates from other geographic origins, the three kinds of virus were detected by RT-PCR in 64 grape samples from Xinjiang and sequenced. The results showed that the positive percentage with GRSPaV, GFkV and GVA were 31.3%, 62.5% and 25.0%, respectively. The GRSPaV isolate (KJ801847) from Xinjiang shared 96.59% identity with USA isolate (AY368590), and GFkV isolate (KJ801846) shared sequence similarity of 91.70% and 91.03% with Japan isolate (AB222861) and Liaoning (China) isolate (JF927942), respectively. GVA isolate (KJ801845) shared 93.88%, 92.92% and 89.53% identity with Poland isolate (JN860997), Sichuan (China) isolate (HQ671655) and Liaoning (China) isolate (FJ445220), respectively. The detection demonstrated that the three grapevine viruses were widespread distributed in Xinjiang with a significant difference from other isolates in China.