| 白菜类作物ITS序列分析 |
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| 引用本文： | 高玉梅,张淑江,章时蕃,李菲,王晓武,武剑,孙日飞.白菜类作物ITS序列分析[J].中国蔬菜,2009,1(24):13-15. |
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| 作者姓名： | 高玉梅 张淑江 章时蕃 李菲 王晓武 武剑 孙日飞 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京100081 |
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| 基金项目： | 国家科技支撑计划，公益性行业(农业)科研专项，农业部蔬菜遗传与生理重点开放实验室资助项目 |
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| 摘 要： | 以不同地理来源的18份不同类型的白菜类作物（Brassica campestris L.）为试材，对其ITS区进行扩增、测序和序列比对，构建NJ系统树，研究白菜类作物ITS序列的变异情况。结果表明：白菜类作物中不同类型材料的ITS序列没有明显变异，序列非常保守；白菜类作物与芸薹属其他种间存在明显变异。芸薹属中的基本种内的变种间没有明显变异；复合种内的变种间有明显变异，分别倾向于禹氏三角（U-triangle）假说推测的父母本。
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| 关 键 词： | 芸薹属 nrDNA 内转录间隔区 遗传距离 |
| 收稿时间： | 2009-05-13; |
ITS Analysis of Brassica rapa |
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| GAO Yu-mei,ZHANG Shu-jiang,ZHANG Shi-fan,LI Fei,WANG Xiao-wu,WU Jian,SUN Ri-fei.ITS Analysis of Brassica rapa[J].China Vegetables,2009,1(24):13-15. |
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| Authors: | GAO Yu-mei ZHANG Shu-jiang ZHANG Shi-fan LI Fei WANG Xiao-wu WU Jian SUN Ri-fei |
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| Institution: | Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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| Abstract: | The ITS sequences of 18 different types of Brassica rapa were amplified by PCR and sequenced,and then the NJ tree was constructed by ITS sequences to analyze the variations of ITS sequence.The results show that the sequences of the ITS of Brassica rapa are very conservative,and no variations exist between different types.Brassica rapa has variations with other species of Brassica respectively.The ITS sequences have variations among the species of Brassica,and the different type materials of the basic species of Brassica have no variations.However,those of the complicate species have variations and the ITS sequences tend to the putative parents of the U-triangle respectively. |
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| Keywords: | nrDNA |
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