| 七子花愈伤组织诱导初探 |
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| 引用本文： | 刘鹏,徐根娣,张杰. 七子花愈伤组织诱导初探[J]. 北京林业大学学报, 2001, 23(6): 63-65 |
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| 作者姓名： | 刘鹏 徐根娣 张杰 |
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| 作者单位： | 浙江师范大学生命与环境科学学院 |
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| 摘 要： | 对七子花愈伤组织的培养途径作了初步的探索 ,研究了以七子花的越冬芽及嫩叶诱导的愈伤组织、无菌短枝扦插和叶柄培养 3条组织培养的途径来进行的七子花种质资源保护 .结果表明 :作为外植体 ,栽培的七子花叶片较野生的七子花越冬芽好 ;以MS +2 ,4 D2 +ZT2培养基最利于幼叶的愈伤组织诱导 ;短枝无菌扦插培养的存活率高于叶柄培养 .比较和分析了各条途径的优点及存在的问题 ,并对七子花的组织培养作了新的展望
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| 关 键 词： | 七子花 组织培养 愈伤组织 种质资源保护 |
Preliminary studies on callus inducement of %Heptacodium miconioides% Rehd. |
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| Liu Peng, Xu Gendi, Zhang Jie.College of Life and Environment Science,Zhejiang Normal Univ.,,P.R.China.. Preliminary studies on callus inducement of %Heptacodium miconioides% Rehd.[J]. Journal of Beijing Forestry University, 2001, 23(6): 63-65 |
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| Authors: | Liu Peng Xu Gendi Zhang Jie.College of Life Environment Science Zhejiang Normal Univ. P.R.China. |
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| Affiliation: | Liu Peng, Xu Gendi, Zhang Jie.College of Life and Environment Science,Zhejiang Normal Univ.,321004,P.R.China. |
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| Abstract: | The avenues of the callus tissue culture of %Heptacodium miconioides% Rehd. were preliminarily explored in this paper. Three relevant ways which could be used in the protection of the germplasm resource of %Heptacodium miconioides% Rehd.were studied (inducing callus with overwintering buds and tender leaves, bacteria|free brachyblast cutting and leafstalk culture). The result showed that tender leaves of cultured %Heptacodium miconioides% were better than overwintering buds of wild %Heptacodium miconioides% as explants of callus;MS+2,4|D2+ZT2 culture medium was the most beneficial medium to callus inducement of tender leaves; survival rate of bacteria|free brachyblast cutting culture was higher than that of leafstalk culture.The advantages and disadvantages of each way were analyzed. This study showed the great potentiality of the tissue culture of %Heptacodium miconioides% Rehd. |
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| Keywords: | Heptacodium miconioides Rehd. tissue culture callus protection of germ plasm resource |
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