药食植物鱼腥草对亚硝化反应抑制作用的研究

在固液比1∶10,提取温度80℃,提取时间60 min条件下,研究五种溶剂提取鱼腥草活性物质中的总黄酮和总酚含量及其对亚硝胺合成的阻断作用与对亚硝酸钠的清除效果。结果表明,55%丙酮提取鱼腥草活性物质中的总黄酮和总酚含量最高,对亚硝胺合成的阻断作用与对亚硝酸钠的清除效果最好,其最佳浓度下提取的鱼腥草活性物质对亚硝胺合成的阻断率达92.81%,对亚硝酸钠的清除率达89.53%;当提取物中的总黄酮含量和VC溶液浓度均为2 mg/mL时,二者对亚硝胺的阻断率分别为51.08%和66.91%,对亚硝酸钠的清除率分别为23.04%和38.22%。

Study on Inhibition of Nitrosation by Extracts of Food and Medicine Plant Houttuynia cordata Thunb.

This paper studied the content of total flavonoids and total phenols in Houttuynia cordata Thunb.active substance extracted by five different solvents,when the solid-to-liquid ration was 1∶10,extraction temperature was 80 ℃,extraction time was 60 minutes.And their blocking effects on the formation of NDMA and scavenging effects on sodium nitrite were studied.The results showed that,the contents of total flavonoids and total phenols in Houttuynia cordata Thunb.active substance was the highest which extracted by 55% acetone,and with the optimum concentration of acetone,the blocking rate of NDMA of Houttuynia cordata Thunb.active substance could reach to 92.81%,and clearance rate of sodium nitrite could reach to 89.53%.When the content of total flavonoids of extract and concentration of VC solution were both 2 mg/mL,their blocking rate of NDMA were 51.08% and 66.91% respectively,and their clearance rate of sodium nitrite were 23.04% and 38.22% respectively.