Leaf surface expansion on the main axes of white and red clovers

Plants of white clover ( Trifolium repens L.) cv. Blanca and red clover ( T. pratense L.) cv. Hungaropoly were grown singly in controlled environments. The effects of eight treatments on the two species were examined: 16- and 8-h photo-periods × 20/15 and 15/10°C day/night temperatures × Rhizobium-free plants receiving nitrate N and inoculated plants receiving no combined N. Twice weekly measurements of the main axis leaf size, petiole length, rate of leaf production and the time period between the appearance of a leaf and its final size were carried out for 8 weeks after sowing.
Increasing the day/night temperature from 15/10 to 20/15°C, or doubling the daylength from 8 to 16 h (doubling the daylength increased temperature by 1·2°C) accelerated the rate of leaf production and expansion and increased petiole length and the final area of leaves. Most aspects of main axis leaf growth were reduced in the inoculated plants dependent on their own N fixation compared with the nodule-free plants receiving abundant nitrate N. The results suggest that the temperature above which white clover exhibits appreciable leaf growth in the field could be relatively high compared with grasses. Further research is required to define these differences and relate them to seasonal changes in the environment.