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Biochemical and morphological characterization of potato clones differing in their resistance to late blight
Authors:D. Evers  N. Welschbillig  J. Dommes  J. F. Hausman
Affiliation:(1) CREBS, CRP-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Grand-Duchy of Luxembourg;(2) Laboratory of Plant Molecular Biology, University of Liège, B-22, Sart Tilman, B-4000 Liège, Belgium
Abstract:Summary Morphological and biochemical parameters that could be involved in resistance to late blight were studied in non-infected and in infected potato hybrids resulting from a cross betweenSolanum phureja, resistant toPhytophthora infestans, and a susceptibleSolanum tuberosum. Some morphological differences between resistant and susceptible hybrids, indicating a positive correlation between stem diameter and phloem thickness in the stem and resistance toP. infestans, were observed. The lignin content in the leaves of the resistant hybrid rose upon infection byP. infestans. In the leaves of the susceptible hybrid, a diminution of the lignin content could be observed upon infection byP. infestans. In the same context, peroxidasic activity raised upon infection byP. infestans in both resistant and susceptible hybrids. Further characterization of the hybrid clones based on the polymorphism of peroxidases was attempted using isoelectric focusing.
Keywords:histology  lignin  isoperoxidases   Phytophthora infestans   potato   Solarium phureja    Solanum tuberosum
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