正交试验法优化酶辅助提取羊栖菜多酚工艺

【目的】采用正交试验法优化羊栖菜多酚的酶辅助提取工艺,以提高羊栖菜多酚提取量,为羊栖菜多酚的提取应用于实际生产提供科学参考。【方法】以新鲜羊栖菜为原料,用酶辅助提取法提取其多酚,通过单因素试验研究纤维素酶添加量、复合酶质量比(中性蛋白酶添加量∶纤维素酶添加量)、酶解温度、酶解pH和酶解时间对多酚提取效果的影响,用正交试验法优化提取工艺条件,并与传统的溶剂提取法进行比较。【结果】各因素对羊栖菜多酚提取量的影响大小依次为:酶解pH>酶解温度>复合酶质量比>酶解时间,其中酶解pH和酶解温度对羊栖菜多酚提取量的影响显著(P<0.05);最佳酶解条件为:酶解温度50℃、酶解pH 5.5、酶解时间45 min、复合酶质量比20∶1(复合酶添加量126 mg/g),在此条件下得到羊栖菜多酚提取量为9.26 mg/g,较溶剂提取法的多酚提取量(8.26 mg/g)有明显提高。【结论】采用正交试验法优化的酶辅助提取工艺能有效提高羊栖菜多酚提取量,优化的工艺参数可在实际生产中加以应用。

Optimization of enzyme-assisted extraction process of polyphenols from Sargassum fusiforme by orthogonal experimental method

Objective]In order to provide scientific reference for extraction and application of polyphenols from Sar-gassum fusiforme, the enzyme-assisted extraction process of polyphenols from S. fusiforme was optimized by orthogonal ex-perimental method to improve yield of polyphenols. Method]With fresh S. fusiforme as raw material, the polyphenols were extracted from S. fusiforme by enzyme-assisted extraction method. The effects of compound enzyme mass ratio(neutral pro-tease amount: cellulase amount), enzymolysis temperature, enzymolysis pH and enzymolysis time on extraction yield of polyphenols were studied through single factor experiment, and then extraction process conditions were optimized by or-thogonal experimental method. The enzyme-assisted extraction method was compared with traditional solvent extraction method. Result]The results showed that, the factors influencing extraction yield of polyphenols were in order as follows:enzymolysis pH>enzymolysis temperature>compound enzyme mass ratio>enzymolysis time. And enzymolysis pH and tem-perature had significant effects on extraction yield of polyphenols(P<0.05). The optimum enzymolysis conditions were as follows: enzymolysis temperature of 50 ℃, enzymolysis pH of 5.5, enzymolysis time of 45 min, and compound enzyme mass ratio of 20∶1(126 mg/g composite enzyme). Under above optimum conditions, the extraction yield of polyphenols from S. fusiforme was up to 9.26 mg/g, and obviously higher than that(8.26 mg/g) of solvent extraction method. Conclusion]Enzyme-assisted extraction process optimized by orthogonal experiment method can effectively improve extraction yield of polyphenols from S. fusiforme, and the optimized process parameters can be applied in actual production.