3种东方百合离体培养

【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是:75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS+0.5 mg/L 6-BA+0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS+0.1 mg/L IBA是生根培养的最佳培养基,提伯、索邦和西伯利亚的生根率分别为96.33%、92.67%和93.67%;移栽的最佳基质为泥炭∶珍珠岩(体积比)=2∶1,提伯、索邦和西伯利亚的移栽成活率分别为97.34%、98.00%和100.00%。【结论】建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产。

In vitro culture for 3 varieties of oriental lily

Objective]The in vitro tissue culture technique of oriental lily was studied, and the unified culture medi-um was screened, in order to provide theoretical and technical support for industrialized seedling production. [Method]The bulb scales of three lily cultivars viz., Tiber, Sorbonne and Siberi were used as explants, which were cultured in minimal medium(MS) supplemented with different hormones. [Result]The results showed that, the best sterilization method was as follow: 0.1% HgCl2 for 12 min, followed by 75% alcohol for 15 s. The suitable culture medium for callus induction and differentiation was MS. The optimum medium for subculture multiplication was MS+0.5 mg/L6-BA+0.5 mg/L NAA , and the multiplication coefficients of Tiber, Sorbonne and Siberi were 3.32, 3.32 and 3.00, respectively. The optimum culture medium for rooting culture was MS+0.1 mg/L IBA, and the rooting rates of Tiber, Sorbonne and Siberi were 96.33%, 92.67%, 93.67% respectively. The optimum substrate for transplanting was peat mixed with perlite in the volume propor-tion of 2∶1, the survival rates of Tiber, Sorbonne and Siberi were 97.34%, 98.00% and 100.00%, respectively. [Conclu-sion]The tissue culture and rapid propagation system for 3 varieties of oriental lily is established , and which is applicable to factory production of lily bulb.