Phylogenetic analyses of plant-growth-promoting rhizobacteria isolated from tomato,lettuce, and Japanese pepper plants in Hyogo Prefecture,Japan |
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Authors: | Tomohiro Kumatani Yukihiko Yoshimi Hitoshi Nakayashiki Masataka Aino |
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Institution: | (1) Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;(2) Research department, Taki Chemical Co., Ltd., 64-1 Nishiwaki, Befu-cho, Kakogawa Hyogo, 675-0125, Japan;(3) Plant Protection Department, Agricultural Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry, and Fisheries, 1533, Minaminooka-koh, Befu-cho, Kasai Hyogo, 679-0198, Japan |
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Abstract: | Approximately 30,000 fluorescent bacterial strains isolated from tomato, lettuce, eggplant, Chinese cabbage, and Japanese
pepper plants at seven different locations in Hyogo Prefecture, were screened for plant-growth-promoting (PGP) activity to
induce disease resistance against bacterial wilt in tomato. The 37 strains that had higher PGP activity were subjected to
molecular phylogenetic analyses using the sequences of the 16S rRNA, gyrB and rpoD genes. Most of the strains were identified as Pseudomonas fluorescens or its close relative, P. putida, while a few strains were grouped with more distantly related bacterial species such as Enterobacter and Stenotrophomonas. The phylogenetic relationships among tomato and lettuce isolates mostly coincided with the source locality and host plants,
with a few exceptions. In contrast, isolates from Japanese pepper plants did not form their own cluster but represented several
different bacterial species. |
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Keywords: | Plant-growth-promoting rhizobacteria Pseudomonas fluorescens 16S RNA rpoD gyrB Phylogeny |
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